Sol Genomics Network

SGN-M6739
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	301869 (SGN) 121297 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U197655	-	-	-
Lycopersicon Combined	Multiple	SGN-U214847	-	-	-
Solanum tuberosum	Single	SGN-U243945	-	-	-
Arabidopsis	Single	At1g29150 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39436

Forward primer (5'-3')

ACCGCATCCTTGAGAACCCTTC

Accessions and product sizes

Approximate temperature

Unknown

Reverse primer (5'-3')

AACATCATCAGCCTGGCTGACC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10131
Forward primer (5'-3') AGGCTACAACCCCGTCTGAGGC		Mg⁺² concentration 3mM
Reverse primer (5'-3') TGATGAAGGGTTCTCAAGGATGCG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 100	Approximate temperature 55°C

PCR data Exp. ID 10132
Forward primer (5'-3') AGGCTACAACCCCGTCTGAGGC		Mg⁺² concentration 3mM
Reverse primer (5'-3') TGATGAAGGGTTCTCAAGGATGCG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 100	Approximate temperature 55°C

PCR data Exp. ID 10133
Forward primer (5'-3') AGGCTACAACCCCGTCTGAGGC		Mg⁺² concentration 3mM
Reverse primer (5'-3') TGATGAAGGGTTCTCAAGGATGCG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 100	Approximate temperature 55°C

PCR data Exp. ID 12172
Forward primer (5'-3') ACCGCATCCTTGAGAACCCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACATCATCAGCCTGGCTGACC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 700	Approximate temperature 55°C

PCR data Exp. ID 12173
Forward primer (5'-3') ACCGCATCCTTGAGAACCCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACATCATCAGCCTGGCTGACC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 700	Approximate temperature 55°C

PCR data Exp. ID 12174
Forward primer (5'-3') ACCGCATCCTTGAGAACCCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACATCATCAGCCTGGCTGACC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 700	Approximate temperature 55°C

PCR data Exp. ID 12175
Forward primer (5'-3') ACCGCATCCTTGAGAACCCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACATCATCAGCCTGGCTGACC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 700	Approximate temperature 55°C

PCR data Exp. ID 12176
Forward primer (5'-3') ACCGCATCCTTGAGAACCCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACATCATCAGCCTGGCTGACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 750+700	Approximate temperature 55°C

PCR data Exp. ID 12177
Forward primer (5'-3') ACCGCATCCTTGAGAACCCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACATCATCAGCCTGGCTGACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 750+700	Approximate temperature 55°C

PCR data Exp. ID 12178
Forward primer (5'-3') ACCGCATCCTTGAGAACCCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACATCATCAGCCTGGCTGACC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 750	Approximate temperature 55°C

PCR data Exp. ID 12179
Forward primer (5'-3') ACCGCATCCTTGAGAACCCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACATCATCAGCCTGGCTGACC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 750	Approximate temperature 55°C

PCR data Exp. ID 12180
Forward primer (5'-3') ACCGCATCCTTGAGAACCCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACATCATCAGCCTGGCTGACC	Enzymes
Accessions and product sizes Physalis TA1367: 700	Approximate temperature 55°C

PCR data Exp. ID 12181
Forward primer (5'-3') ACCGCATCCTTGAGAACCCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACATCATCAGCCTGGCTGACC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 750+700	Approximate temperature 55°C

PCR data Exp. ID 12182
Forward primer (5'-3') ACCGCATCCTTGAGAACCCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACATCATCAGCCTGGCTGACC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 750+700	Approximate temperature 55°C

PCR data Exp. ID 12183
Forward primer (5'-3') ACCGCATCCTTGAGAACCCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACATCATCAGCCTGGCTGACC	Enzymes
Accessions and product sizes Coffea canephora BP409: 750	Approximate temperature 55°C

PCR data Exp. ID 33310
Forward primer (5'-3') ACCGCATCCTTGAGAACCCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACATCATCAGCCTGGCTGACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 750+700	Approximate temperature 55°C

PCR data Exp. ID 33311
Forward primer (5'-3') ACCGCATCCTTGAGAACCCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACATCATCAGCCTGGCTGACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 750+700	Approximate temperature 55°C

PCR data Exp. ID 33312
Forward primer (5'-3') ACCGCATCCTTGAGAACCCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACATCATCAGCCTGGCTGACC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 750	Approximate temperature 55°C

PCR data Exp. ID 33313
Forward primer (5'-3') ACCGCATCCTTGAGAACCCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACATCATCAGCCTGGCTGACC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 750	Approximate temperature 55°C