Sol Genomics Network

SGN Marker C2_At1g53000

SGN-M6856
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	304055 (SGN) 124971 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U201101	-	-	-
Lycopersicon Combined	Single	SGN-U216303	-	-	-
Solanum tuberosum	Single	SGN-U263773	-	-	-
Arabidopsis	Single	At1g53000 on TAIR	-	-	-

Mapped locations

Map: Tomato-EXPEN 2000
Map version 52 (location: 38200)
Chromosome 10
Position 7.50 cM
Confidence F(LOD3)
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39478

Forward primer (5'-3')

AGATTCTCGGCAAGCCTATGATCC

Accessions and product sizes

Solanum lycopersicum TA209

350

Solanum pennellii TA56

350

Approximate temperature

55°C

Reverse primer (5'-3')

AAGCTTTGCCCTTTCCCATGTTC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

350

Solanum pennellii TA56

300

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25265)
Chromosome 1
Position 19.75 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10264
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1800	Approximate temperature 55°C

PCR data Exp. ID 10265
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1800	Approximate temperature 55°C

PCR data Exp. ID 10266
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1800	Approximate temperature 55°C

PCR data Exp. ID 12691
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12692
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Solanum melongena TA2986: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12693
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12694
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1200	Approximate temperature 55°C

PCR data Exp. ID 12695
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 350+1100	Approximate temperature 55°C

PCR data Exp. ID 12696
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1200	Approximate temperature 55°C

PCR data Exp. ID 12697
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1200	Approximate temperature 55°C

PCR data Exp. ID 12698
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 1200	Approximate temperature 55°C

PCR data Exp. ID 12699
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Physalis TA1367: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12700
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 600+900	Approximate temperature 55°C

PCR data Exp. ID 12701
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 550+850	Approximate temperature 55°C

PCR data Exp. ID 12702
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 550+750	Approximate temperature 55°C

PCR data Exp. ID 12703
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 600+800	Approximate temperature 55°C

PCR data Exp. ID 12704
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Coffea canephora BP409: 350	Approximate temperature 55°C

PCR data Exp. ID 32794
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1200	Approximate temperature 55°C

PCR data Exp. ID 32795
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 350+1100	Approximate temperature 55°C

PCR data Exp. ID 32796
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1200	Approximate temperature 55°C

PCR data Exp. ID 32797
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1200	Approximate temperature 55°C

PCR data Exp. ID 10267
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC	Digested band sizes (using DpnII) Solanum pennellii LA716: 650 Solanum lycopersicum LA925: 500+700	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes All possible enzymes for pennellii and and lycopersicum: TaqI,DpnII All possible enzymes for lycopersicum and pimpinellifolium: DpnII
Accessions and product sizes Solanum pennellii LA716: 1800 Solanum lycopersicum LA925: 1800	Approximate temperature 55°C

Unigene blast matches for primers

SGN-U216303

Universal primers for Asterid species

No additional PCR data found.

Other COSII sequence data

BLASTX result of original unigene sequences against Arabidopsis protein database

Alignment of Arabidopsis CDS and edited Asterid unigenes, FASTA
Alignment of DNA and translated peptides from Arabidopsis CDS and edited Asterid unigenes, plain text
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, ClustalW
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, FASTA

BLASTX result of original unigene sequences against Arabidopsis protein database

Related Markers

Related Genotyped Markers: These markers have been genotyped and share a name with this marker

Genomic location of C2_At1g53000

%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D6856 marker 6856

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Accessions	Seedlots
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