Sol Genomics Network

SGN Marker C2_At1g63980

SGN-M6940
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	301269 (SGN) 122209 (CGN)	-	-	-
Lycopersicon Combined	Multiple	SGN-U212644	-	-	-
Solanum tuberosum	Single	SGN-U245770	-	-	-
Arabidopsis	Single	At1g63980 on TAIR	-	-	-

Mapped locations

Map: Pepper-COSII
Map version 58 (location: 41340)
Chromosome 1
Position 0.00 cM
Confidence F(LOD3)
Protocol dCAPS

Pepper-COSII

PCR data Exp. ID 43316

Forward primer (5'-3')

TGATTTAATCATTGTCATTTTAGTTCTA

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

TGAGCATTTACAAGCGTATACGAAA

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38228)
Chromosome 8
Position 77.00 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39500

Forward primer (5'-3')

AGAAAGTCTGCCGCCTTCCG

Accessions and product sizes

Solanum pennellii TA56

1650

Solanum lycopersicum TA209

1650

Approximate temperature

50°C

Reverse primer (5'-3')

TAGGCCTTCTCCTTCTTCCCATC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

650

Solanum pennellii TA56

670

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25353)
Chromosome 1
Position 23.75 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10339
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1650	Approximate temperature 50°C

PCR data Exp. ID 10340
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1650	Approximate temperature 50°C

PCR data Exp. ID 10341
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1650	Approximate temperature 50°C

PCR data Exp. ID 12769
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 1400	Approximate temperature 55°C

PCR data Exp. ID 12770
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 1000	Approximate temperature 55°C

PCR data Exp. ID 12771
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 1350	Approximate temperature 55°C

PCR data Exp. ID 12772
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1050	Approximate temperature 55°C

PCR data Exp. ID 12773
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 900	Approximate temperature 55°C

PCR data Exp. ID 12774
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 900	Approximate temperature 55°C

PCR data Exp. ID 12775
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 900	Approximate temperature 55°C

PCR data Exp. ID 12776
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Physalis TA1367: 1500	Approximate temperature 55°C

PCR data Exp. ID 12777
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 1000	Approximate temperature 55°C

PCR data Exp. ID 32606
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1650	Approximate temperature 55°C

PCR data Exp. ID 32829
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1650	Approximate temperature 55°C

PCR data Exp. ID 32830
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1650	Approximate temperature 55°C

PCR data Exp. ID 32831
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1050	Approximate temperature 55°C

PCR data Exp. ID 32832
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 900	Approximate temperature 55°C

PCR data Exp. ID 32833
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 900	Approximate temperature 55°C

PCR data Exp. ID 10342
Forward primer (5'-3') AGAAAGTCTGCCGCCTTCCG	Digested band sizes (using HinfI) Solanum lycopersicum LA925: 650 Solanum pennellii LA716: 670	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TAGGCCTTCTCCTTCTTCCCATC	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI, MspI, DraI, EcoRV, HaeIII, HinfI, DpnII, KpnI
Accessions and product sizes Solanum lycopersicum LA925: 1650 Solanum pennellii LA716: 1650	Approximate temperature 50°C