Sol Genomics Network

SGN-M6965
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	300438 (SGN) 119781 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U196561	-	-	-
Lycopersicon Combined	Single	SGN-U218032	-	-	-
Solanum tuberosum	Single	SGN-U245533	-	-	-
Arabidopsis	Single	At1g65720 on TAIR	-	-	-

Pepper-COSII

PCR data Exp. ID 43319

Forward primer (5'-3')

TCGCTTTCTCTCTCCTCGCCGTCTGCG

Accessions and product sizes

Approximate temperature

58°C

Reverse primer (5'-3')

TCGCAGCAGTTAACGCACCACAACC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Tomato-EXPEN 2000

PCR data Exp. ID 39509

Forward primer (5'-3')

TTCTACTTCTCGCTTTCTCTCTCCTC

Accessions and product sizes

Solanum lycopersicum TA209

1500

Solanum pennellii TA56

1500

Approximate temperature

55°C

Reverse primer (5'-3')

TCGCAGCAGTTAACGCACCACAACC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10356
Forward primer (5'-3') TTCTACTTCTCGCTTTCTCTCTCCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCAGCAGTTAACGCACCACAACC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 500	Approximate temperature 55°C

PCR data Exp. ID 10357
Forward primer (5'-3') TTCTACTTCTCGCTTTCTCTCTCCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCAGCAGTTAACGCACCACAACC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 500	Approximate temperature 55°C

PCR data Exp. ID 10358
Forward primer (5'-3') TTCTACTTCTCGCTTTCTCTCTCCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCAGCAGTTAACGCACCACAACC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 500	Approximate temperature 55°C