Sol Genomics Network

SGN Marker C2_At1g67730

SGN-M6986
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	300528 (SGN) 123973 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U197944	-	-	-
Lycopersicon Combined	Single	SGN-U215119	-	-	-
Solanum tuberosum	Single	SGN-U246038	-	-	-
Arabidopsis	Single	At1g67730 on TAIR	-	-	-

Mapped locations

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46606)
Chromosome 12
Position 28.80 cM
Confidence CF(LOD3)
Protocol CAPS

Tobacco N. tomentosiformis

Map: Pepper-COSII
Map version 58 (location: 41345)
Chromosome 2
Position 141.60 cM
Confidence F(LOD3)
Protocol PCR

Pepper-COSII

PCR data Exp. ID 43321

Forward primer (5'-3')

TGGGATTGATGTGCAATGCCAGG

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

AGGGCAGCCCGAGCATAACC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38244)
Chromosome 2
Position 143.00 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39515

Forward primer (5'-3')

TGGGATTGATGTGCAATGCCAGG

Accessions and product sizes

Solanum lycopersicum TA209

750

Solanum pennellii TA56

750

Approximate temperature

55°C

Reverse primer (5'-3')

AGGGCAGCCCGAGCATAACC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

600

Solanum pennellii TA56

750

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25399)
Chromosome 1
Position 25.40 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10376
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 750	Approximate temperature 55°C

PCR data Exp. ID 10377
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 750	Approximate temperature 55°C

PCR data Exp. ID 10378
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 750	Approximate temperature 55°C

PCR data Exp. ID 12227
Forward primer (5'-3') ACCTGCTAAGAATCTTAAGAAATATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATACAGAGGATCAGAGGGGATAAC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 500	Approximate temperature 55°C

PCR data Exp. ID 12228
Forward primer (5'-3') ACCTGCTAAGAATCTTAAGAAATATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATACAGAGGATCAGAGGGGATAAC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 500	Approximate temperature 55°C

PCR data Exp. ID 12229
Forward primer (5'-3') ACCTGCTAAGAATCTTAAGAAATATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATACAGAGGATCAGAGGGGATAAC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 500	Approximate temperature 55°C

PCR data Exp. ID 12230
Forward primer (5'-3') ACCTGCTAAGAATCTTAAGAAATATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATACAGAGGATCAGAGGGGATAAC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 500	Approximate temperature 55°C

PCR data Exp. ID 12231
Forward primer (5'-3') ACCTGCTAAGAATCTTAAGAAATATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATACAGAGGATCAGAGGGGATAAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 500	Approximate temperature 55°C

PCR data Exp. ID 12232
Forward primer (5'-3') ACCTGCTAAGAATCTTAAGAAATATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATACAGAGGATCAGAGGGGATAAC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 500	Approximate temperature 55°C

PCR data Exp. ID 12233
Forward primer (5'-3') ACCTGCTAAGAATCTTAAGAAATATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATACAGAGGATCAGAGGGGATAAC	Enzymes
Accessions and product sizes Physalis TA1367: 500	Approximate temperature 55°C

PCR data Exp. ID 12234
Forward primer (5'-3') ACCTGCTAAGAATCTTAAGAAATATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATACAGAGGATCAGAGGGGATAAC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 500	Approximate temperature 55°C

PCR data Exp. ID 12235
Forward primer (5'-3') ACCTGCTAAGAATCTTAAGAAATATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATACAGAGGATCAGAGGGGATAAC	Enzymes
Accessions and product sizes Coffea canephora BP409: 500	Approximate temperature 55°C

PCR data Exp. ID 12787
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 700	Approximate temperature 55°C

PCR data Exp. ID 12788
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 800	Approximate temperature 55°C

PCR data Exp. ID 12789
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 920	Approximate temperature 55°C

PCR data Exp. ID 12790
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1200	Approximate temperature 55°C

PCR data Exp. ID 12791
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 1200	Approximate temperature 55°C

PCR data Exp. ID 12792
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1150	Approximate temperature 55°C

PCR data Exp. ID 12793
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1100	Approximate temperature 55°C

PCR data Exp. ID 12794
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Physalis TA1367: 650	Approximate temperature 55°C

PCR data Exp. ID 12795
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 300	Approximate temperature 55°C

PCR data Exp. ID 12796
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 250	Approximate temperature 55°C

PCR data Exp. ID 12797
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 300	Approximate temperature 55°C

PCR data Exp. ID 32838
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1200	Approximate temperature 55°C

PCR data Exp. ID 32839
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 1200	Approximate temperature 55°C

PCR data Exp. ID 32840
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1150	Approximate temperature 55°C

PCR data Exp. ID 32841
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1100	Approximate temperature 55°C

PCR data Exp. ID 33329
Forward primer (5'-3') ACCTGCTAAGAATCTTAAGAAATATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATACAGAGGATCAGAGGGGATAAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 500	Approximate temperature 55°C

PCR data Exp. ID 33330
Forward primer (5'-3') ACCTGCTAAGAATCTTAAGAAATATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATACAGAGGATCAGAGGGGATAAC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 500	Approximate temperature 55°C

PCR data Exp. ID 10379
Forward primer (5'-3') TGGGATTGATGTGCAATGCCAGG	Digested band sizes (using RsaI) Solanum pennellii LA716: 750 Solanum lycopersicum LA925: 600	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGGCAGCCCGAGCATAACC	Enzymes All possible enzymes for pennellii and and lycopersicum: RsaI, DpnII
Accessions and product sizes Solanum lycopersicum LA925: 750 Solanum pennellii LA716: 750	Approximate temperature 55°C

Unigene blast matches for primers

SGN-U215119

Universal primers for Asterid species

No additional PCR data found.

Other COSII sequence data

BLASTX result of original unigene sequences against Arabidopsis protein database

Alignment of Arabidopsis CDS and edited Asterid unigenes, FASTA
Alignment of DNA and translated peptides from Arabidopsis CDS and edited Asterid unigenes, plain text
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, ClustalW
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, FASTA

Input file for PAUP, NEXUS format
Phylogenetic tree
Phylogenetic tree | [View]
Forward amplicon sequence for S. hirtum 130005 plant #3, AB1 chromatogram - [View]
Forward amplicon sequence for S. hirtum 130005 plant #3, plain text
Forward amplicon sequence for S. hirtum 120062 plant #28, AB1 chromatogram - [View]
Forward amplicon sequence for S. hirtum 120062 plant #28, plain text
Forward amplicon sequence for S. quitoense plant #3, AB1 chromatogram - [View]
Forward amplicon sequence for S. quitoense plant #3, plain text
Forward amplicon sequence for S. quitoense SE plant #1, AB1 chromatogram - [View]
Forward amplicon sequence for S. quitoense SE plant #1, plain text

BLASTX result of original unigene sequences against Arabidopsis protein database

Related Markers

Related Genotyped Markers: These markers have been genotyped and share a name with this marker

Genomic location of C2_At1g67730

%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D6986 marker 6986

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Accessions	Seedlots
Accessions		Breeding Program	Seedlot Name	Contents	Seedlot Location	Count	Weight(g)

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