Sol Genomics Network

SGN-M7051
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	309682 (SGN) 132513 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U201629	-	-	-
Lycopersicon Combined	Single	SGN-U221108	-	-	-
Solanum tuberosum	Single	SGN-U254562	-	-	-
Arabidopsis	Single	At1g74640 on TAIR	-	-	-

Arabidopsis COSII

PCR data Exp. ID 41163

Forward primer (5'-3')

ACCTAAGCTTGATTACAATGCTGATG

Accessions and product sizes

Solanum pennellii TA56

2500

Solanum lycopersicum TA209

2400

Approximate temperature

55°C

Reverse primer (5'-3')

TGCTAAGGTAGCAGCATGTCCTCC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Other PCR data

PCR data Exp. ID 10419
Forward primer (5'-3') ACCTAAGCTTGATTACAATGCTGATG		Mg⁺² concentration 3mM
Reverse primer (5'-3') TGCTAAGGTAGCAGCATGTCCTCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 2500	Approximate temperature 55°C

PCR data Exp. ID 10420
Forward primer (5'-3') ACCTAAGCTTGATTACAATGCTGATG		Mg⁺² concentration 3mM
Reverse primer (5'-3') TGCTAAGGTAGCAGCATGTCCTCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 2400	Approximate temperature 55°C

PCR data Exp. ID 10421
Forward primer (5'-3') ACCTAAGCTTGATTACAATGCTGATG		Mg⁺² concentration 3mM
Reverse primer (5'-3') TGCTAAGGTAGCAGCATGTCCTCC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 2400	Approximate temperature 55°C

PCR data Exp. ID 12807
Forward primer (5'-3') ACCTAAGCTTGATTACAATGCTGATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTAAGGTAGCAGCATGTCCTCC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 2400	Approximate temperature 55°C

PCR data Exp. ID 12808
Forward primer (5'-3') ACCTAAGCTTGATTACAATGCTGATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTAAGGTAGCAGCATGTCCTCC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 2600	Approximate temperature 55°C

PCR data Exp. ID 12809
Forward primer (5'-3') ACCTAAGCTTGATTACAATGCTGATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTAAGGTAGCAGCATGTCCTCC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 2500	Approximate temperature 55°C

PCR data Exp. ID 12810
Forward primer (5'-3') ACCTAAGCTTGATTACAATGCTGATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTAAGGTAGCAGCATGTCCTCC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1300	Approximate temperature 55°C

PCR data Exp. ID 12811
Forward primer (5'-3') ACCTAAGCTTGATTACAATGCTGATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTAAGGTAGCAGCATGTCCTCC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 900	Approximate temperature 55°C

PCR data Exp. ID 12812
Forward primer (5'-3') ACCTAAGCTTGATTACAATGCTGATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTAAGGTAGCAGCATGTCCTCC	Enzymes
Accessions and product sizes Coffea canephora BP409: 1200	Approximate temperature 55°C

PCR data Exp. ID 32608
Forward primer (5'-3') ACCTAAGCTTGATTACAATGCTGATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTAAGGTAGCAGCATGTCCTCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 2400	Approximate temperature 55°C

PCR data Exp. ID 32846
Forward primer (5'-3') ACCTAAGCTTGATTACAATGCTGATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTAAGGTAGCAGCATGTCCTCC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 2400	Approximate temperature 55°C

PCR data Exp. ID 32847
Forward primer (5'-3') ACCTAAGCTTGATTACAATGCTGATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTAAGGTAGCAGCATGTCCTCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 2500	Approximate temperature 55°C

PCR data Exp. ID 32848
Forward primer (5'-3') ACCTAAGCTTGATTACAATGCTGATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTAAGGTAGCAGCATGTCCTCC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1300	Approximate temperature 55°C