Sol Genomics Network

SGN Marker C2_At2g01720

SGN-M7141
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Multiple	300846 (SGN) 121141 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U200161	-	-	-
Lycopersicon Combined	Single	SGN-U218512	-	-	-
Solanum tuberosum	Single	SGN-U246003	-	-	-
Arabidopsis	Single	At2g01720 on TAIR	-	-	-

Mapped locations

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46624)
Chromosome 5
Position 45.40 cM
Confidence F(LOD3)
Protocol CAPS

Tobacco N. tomentosiformis

Map: Tomato-EXPEN 2000
Map version 52 (location: 38286)
Chromosome 5
Position 119.00 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39551

Forward primer (5'-3')

ACAAATTGGTACATGCTGGTGCTC

Accessions and product sizes

Solanum lycopersicum TA209

1100

Solanum pennellii TA56

1100

Approximate temperature

55°C

Reverse primer (5'-3')

TGGCCTGTTAGACTGATATTCAAC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

1100

Solanum pennellii TA56

950

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25556)
Chromosome 2
Position 0.32 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10501
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 500	Approximate temperature 55°C

PCR data Exp. ID 10502
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 500	Approximate temperature 55°C

PCR data Exp. ID 10503
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 500	Approximate temperature 55°C

PCR data Exp. ID 12899
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 500	Approximate temperature 55°C

PCR data Exp. ID 12900
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 380	Approximate temperature 55°C

PCR data Exp. ID 12901
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 500	Approximate temperature 55°C

PCR data Exp. ID 12902
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 500	Approximate temperature 55°C

PCR data Exp. ID 12903
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 500	Approximate temperature 55°C

PCR data Exp. ID 12904
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 500	Approximate temperature 55°C

PCR data Exp. ID 12905
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Physalis TA1367: 500	Approximate temperature 55°C

PCR data Exp. ID 12906
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Petunia axillaris TA3309: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12907
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 1300	Approximate temperature 55°C

PCR data Exp. ID 12908
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 350+520	Approximate temperature 55°C

PCR data Exp. ID 32617
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 600	Approximate temperature 55°C

PCR data Exp. ID 32890
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 600	Approximate temperature 55°C

PCR data Exp. ID 32891
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 600	Approximate temperature 55°C

PCR data Exp. ID 32892
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 500	Approximate temperature 55°C

PCR data Exp. ID 32893
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 500	Approximate temperature 55°C

PCR data Exp. ID 32894
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 500	Approximate temperature 55°C

PCR data Exp. ID 10504
Forward primer (5'-3') ACAAATTGGTACATGCTGGTGCTC	Digested band sizes (using DraI) Solanum lycopersicum LA925: 250+150 Solanum pennellii LA716: 500	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCCTGTTAGACTGATATTCAAC	Enzymes All possible enzymes for pennellii and and lycopersicum: DraI All possible enzymes for lycopersicum and pimpinellifolium: DraI
Accessions and product sizes Solanum pennellii LA716: 500 Solanum lycopersicum LA925: 500	Approximate temperature 55°C