Sol Genomics Network

SGN-M7179
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Capsicum annuum	Single	SGN-U205516	-	-	-
Lycopersicon Combined	Single	SGN-U219488	-	-	-
Solanum tuberosum	Multiple	SGN-U247736	-	-	-
Arabidopsis	Single	At2g06010 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39560

Forward primer (5'-3')

TGCAGGCAACAGGTTGGAAATGG

Accessions and product sizes

Solanum lycopersicum TA209

1650

Solanum pennellii TA56

1650

Approximate temperature

53°C

Reverse primer (5'-3')

AGATGGCCTCCACTCTATCCAGTGAC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10531
Forward primer (5'-3') TGCAGGCAACAGGTTGGAAATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGATGGCCTCCACTCTATCCAGTGAC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 400	Approximate temperature 55°C

PCR data Exp. ID 10532
Forward primer (5'-3') TGCAGGCAACAGGTTGGAAATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGATGGCCTCCACTCTATCCAGTGAC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 400	Approximate temperature 55°C

PCR data Exp. ID 10533
Forward primer (5'-3') TGCAGGCAACAGGTTGGAAATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGATGGCCTCCACTCTATCCAGTGAC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 400	Approximate temperature 55°C

PCR data Exp. ID 10534
Forward primer (5'-3') TGCAGGCAACAGGTTGGAAATGG	Digested band sizes (using MspI) Solanum lycopersicum LA925: 300 Solanum pennellii LA716: 200+150+50	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGATGGCCTCCACTCTATCCAGTGAC	Enzymes All possible enzymes for pennellii and and lycopersicum: BslI,HinfI,MspI All possible enzymes for lycopersicum and pimpinellifolium: HinfI,MspI
Accessions and product sizes Solanum pennellii LA716: 400 Solanum lycopersicum LA925: 400	Approximate temperature 55°C