Sol Genomics Network

SGN-M7183
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Capsicum annuum	Single	SGN-U201743	-	-	-
Lycopersicon Combined	Single	SGN-U224692	-	-	-
Solanum tuberosum	Single	SGN-U251463	-	-	-
Arabidopsis	Single	At2g06925 on TAIR	-	-	-

Map: Tomato - Kazusa and SolCAP markers mapped to genome
Map version 105 (location: 49226)
Chromosome 7
Position 5.20 MB
Confidence uncalculated
Protocol CAPS

Tomato - Kazusa and SolCAP markers mapped to genome

Kazusa F2-2000 genetic map

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46627)
Chromosome 11
Position 37.90 cM
Confidence CF(LOD3)
Protocol CAPS

Tobacco N. tomentosiformis

Tomato-EXPEN 2000

PCR data Exp. ID 39562

Forward primer (5'-3')

AGAATGCAGTAGAACATGTGAATCAAAG

Accessions and product sizes

Solanum lycopersicum TA209

325

Solanum pennellii TA56

325

Approximate temperature

55°C

Reverse primer (5'-3')

AATCATGCTTCATACAACAAGCATCAAG

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

150

Solanum lycopersicum TA209

325

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10545
Forward primer (5'-3') AGAATGCAGTAGAACATGTGAATCAAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AATCATGCTTCATACAACAAGCATCAAG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 240	Approximate temperature 55°C

PCR data Exp. ID 10546
Forward primer (5'-3') AGAATGCAGTAGAACATGTGAATCAAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AATCATGCTTCATACAACAAGCATCAAG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 240	Approximate temperature 55°C

PCR data Exp. ID 10547
Forward primer (5'-3') AGAATGCAGTAGAACATGTGAATCAAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AATCATGCTTCATACAACAAGCATCAAG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 240	Approximate temperature 55°C

PCR data Exp. ID 10548
Forward primer (5'-3') AGAATGCAGTAGAACATGTGAATCAAAG	Digested band sizes (using ApoI) Solanum lycopersicum LA925: 140+90 Solanum pennellii LA716: 150+90	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AATCATGCTTCATACAACAAGCATCAAG	Enzymes All possible enzymes for pennellii and and lycopersicum: ApoI
Accessions and product sizes Solanum pennellii LA716: 240 Solanum lycopersicum LA925: 240	Approximate temperature 55°C