Sol Genomics Network

SGN-M7194
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	306024 (SGN) 131660 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U198059	-	-	-
Lycopersicon Combined	Single	SGN-U220663	-	-	-
Solanum tuberosum	Single	SGN-U253479	-	-	-
Arabidopsis	Single	At2g14260 on TAIR	-	-	-

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46628)
Chromosome 3
Position 4.70 cM
Confidence F(LOD3)
Protocol CAPS

Tobacco N. tomentosiformis

Tomato-EXPEN 2000

PCR data Exp. ID 39563

Forward primer (5'-3')

AGGATCTATACCCCTCTATAGAGCC

Accessions and product sizes

Solanum pennellii TA56

700

Solanum lycopersicum TA209

700

Approximate temperature

55°C

Reverse primer (5'-3')

TTATTGGGTGAAGTCCCACCTCC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

700

Solanum pennellii TA56

310+390

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10555
Forward primer (5'-3') AGGATCTATACCCCTCTATAGAGCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTATTGGGTGAAGTCCCACCTCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1450	Approximate temperature 55°C

PCR data Exp. ID 10556
Forward primer (5'-3') AGGATCTATACCCCTCTATAGAGCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTATTGGGTGAAGTCCCACCTCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1450	Approximate temperature 55°C

PCR data Exp. ID 10557
Forward primer (5'-3') AGGATCTATACCCCTCTATAGAGCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTATTGGGTGAAGTCCCACCTCC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1450	Approximate temperature 55°C

PCR data Exp. ID 32230
Forward primer (5'-3') AGGATCTATACCCCTCTATAGAGCC	Digested band sizes (using CfoI) Solanum pennellii LA716: 300+400+750 Solanum lycopersicum LA925: 400+1050	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTATTGGGTGAAGTCCCACCTCC	Enzymes All possible enzymes for pennellii and and lycopersicum: BglII,BstNI,BstYI,CfoI/HhaI,ScrFI All possible enzymes for lycopersicum and pimpinellifolium: TspRI
Accessions and product sizes Solanum lycopersicum LA925: 1450 Solanum pennellii LA716: 1450	Approximate temperature 55°C