Sol Genomics Network

SGN-M7201
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	311010 (SGN) 130722 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U201337	-	-	-
Arabidopsis	Single	At2g15320 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39565

Forward primer (5'-3')

ACAAGTTGACATGGGGAATAACAGC

Accessions and product sizes

Solanum pennellii TA56

350

Solanum lycopersicum TA209

350

Approximate temperature

55°C

Reverse primer (5'-3')

AGGTTTCTGTGATTTTAAGCAAAGTTG

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

280

Solanum pennellii TA56

350

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10564
Forward primer (5'-3') ACAAGTTGACATGGGGAATAACAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGTTTCTGTGATTTTAAGCAAAGTTG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 380	Approximate temperature 55°C

PCR data Exp. ID 10565
Forward primer (5'-3') ACAAGTTGACATGGGGAATAACAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGTTTCTGTGATTTTAAGCAAAGTTG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 380	Approximate temperature 55°C

PCR data Exp. ID 10566
Forward primer (5'-3') ACAAGTTGACATGGGGAATAACAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGTTTCTGTGATTTTAAGCAAAGTTG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 380	Approximate temperature 55°C

PCR data Exp. ID 32232
Forward primer (5'-3') ACAAGTTGACATGGGGAATAACAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGTTTCTGTGATTTTAAGCAAAGTTG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 380	Approximate temperature 53°C

PCR data Exp. ID 32233
Forward primer (5'-3') ACAAGTTGACATGGGGAATAACAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGTTTCTGTGATTTTAAGCAAAGTTG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 380	Approximate temperature 53°C

PCR data Exp. ID 32234
Forward primer (5'-3') ACAAGTTGACATGGGGAATAACAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGTTTCTGTGATTTTAAGCAAAGTTG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 380	Approximate temperature 53°C

PCR data Exp. ID 32235
Forward primer (5'-3') ACAAGTTGACATGGGGAATAACAGC	Digested band sizes (using ApoI) Solanum lycopersicum LA925: 270 Solanum pennellii LA716: 380	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGTTTCTGTGATTTTAAGCAAAGTTG	Enzymes All possible enzymes for pennellii and and lycopersicum: ApoI,AvaII,HaeIII
Accessions and product sizes Solanum lycopersicum LA925: 380 Solanum pennellii LA716: 380	Approximate temperature 53°C