SGN Marker C2_At2g20390

SGN-M7247
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

SynonymsNone
Locus associationsNone
Orthologs in this COSII group 
SpeciesCopiesSequence IDCDS/Edited sequencePeptide sequencePredicted introns
CoffeeSingle301933 (SGN)
122888 (CGN)
---
Lycopersicon CombinedSingleSGN-U219413---
Solanum tuberosumSingleSGN-U254177---
ArabidopsisSingleAt2g20390 on TAIR---
Mapped locations  
Map: Tomato-EXPEN 2000   
Map version
52 (location: 38311)
Chromosome
4
Position
27.00 cM
Confidence
I
Protocol
CAPS


Tomato-EXPEN 2000

PCR data   Exp. ID 39573
Forward primer (5'-3')
AGCCCATTGTTAGAGGTCCTTGG
Accessions and product sizes
Approximate temperature
55°C
Reverse primer (5'-3')
ACTTTAATCCGAAAAGTTTGCTGC
Digested band sizes (using unknown enzyme)
Mg+2 concentration
1.5mM
   Enzymes
  

Map: Arabidopsis COSII   
Map version
44 (location: 25662)
Chromosome
2
Position
8.80 MB
Confidence
uncalculated
Protocol
CAPS


Arabidopsis COSII
Other PCR data 
PCR data   Exp. ID 10601
Forward primer (5'-3')
AGCCCATTGTTAGAGGTCCTTGG
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
ACTTTAATCCGAAAAGTTTGCTGC
Enzymes
  
Accessions and product sizes
Solanum pennellii LA716: 1200
Approximate temperature
55°C
  
PCR data   Exp. ID 10602
Forward primer (5'-3')
AGCCCATTGTTAGAGGTCCTTGG
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
ACTTTAATCCGAAAAGTTTGCTGC
Enzymes
  
Accessions and product sizes
Solanum lycopersicum LA925: 1200
Approximate temperature
55°C
  
PCR data   Exp. ID 10603
Forward primer (5'-3')
AGCCCATTGTTAGAGGTCCTTGG
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
ACTTTAATCCGAAAAGTTTGCTGC
Enzymes
  
Accessions and product sizes
Solanum pimpinellifolium LA1589: 1200
Approximate temperature
55°C
  
PCR data   Exp. ID 10604
Forward primer (5'-3')
AGCCCATTGTTAGAGGTCCTTGG
Digested band sizes (using DraI)
Solanum lycopersicum LA925: 410+430
Solanum pennellii LA716: 390+410
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
ACTTTAATCCGAAAAGTTTGCTGC
Enzymes
All possible enzymes for pennellii and and lycopersicum: AluI,BsiEI,BspDI,DraI,HinfI,Hpy188I,RsaI,SsPI,TaqI
All possible enzymes for lycopersicum and pimpinellifolium: BstZ17I,HinfI,Hpy188I
  
Accessions and product sizes
Solanum lycopersicum LA925: 1200
Solanum pennellii LA716: 1200
Approximate temperature
55°C
  
Unigene blast matches for primers 
Universal primers for Asterid species 
No additional PCR data found.
Other COSII sequence data 

Alignment of Arabidopsis CDS and edited Asterid unigenes, FASTA
Alignment of DNA and translated peptides from Arabidopsis CDS and edited Asterid unigenes, plain text
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, ClustalW
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, FASTA

Amplicon sequence alignment, FASTA
At2g20390F.cds.txt, plain text
Forward amplicon sequence for S. pimpinellifolium LA1589, AB1 chromatogram - [View]
Forward amplicon sequence for S. pimpinellifolium LA1589, plain text
Forward amplicon sequence for S. lycopersicum TA209, AB1 chromatogram - [View]
Forward amplicon sequence for S. lycopersicum TA209, plain text
Forward amplicon sequence for S. pennellii TA56, AB1 chromatogram - [View]
Forward amplicon sequence for S. pennellii TA56, plain text
Forward amplicon sequence for S. hirtum 120062 plant #28, AB1 chromatogram - [View]
Forward amplicon sequence for S. hirtum 120062 plant #28, plain text
Forward amplicon sequence for S. quitoense plant #3, AB1 chromatogram - [View]
Forward amplicon sequence for S. quitoense plant #3, plain text

Genomic location of C2_At2g20390  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D7247 marker 7247
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