Sol Genomics Network

SGN-M7247
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	301933 (SGN) 122888 (CGN)	-	-	-
Lycopersicon Combined	Single	SGN-U219413	-	-	-
Solanum tuberosum	Single	SGN-U254177	-	-	-
Arabidopsis	Single	At2g20390 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39573

Forward primer (5'-3')

AGCCCATTGTTAGAGGTCCTTGG

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

ACTTTAATCCGAAAAGTTTGCTGC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10601
Forward primer (5'-3') AGCCCATTGTTAGAGGTCCTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTTAATCCGAAAAGTTTGCTGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1200	Approximate temperature 55°C

PCR data Exp. ID 10602
Forward primer (5'-3') AGCCCATTGTTAGAGGTCCTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTTAATCCGAAAAGTTTGCTGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1200	Approximate temperature 55°C

PCR data Exp. ID 10603
Forward primer (5'-3') AGCCCATTGTTAGAGGTCCTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTTAATCCGAAAAGTTTGCTGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1200	Approximate temperature 55°C

PCR data Exp. ID 10604
Forward primer (5'-3') AGCCCATTGTTAGAGGTCCTTGG	Digested band sizes (using DraI) Solanum pennellii LA716: 410+390 Solanum lycopersicum LA925: 430+410	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTTAATCCGAAAAGTTTGCTGC	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI,BsiEI,BspDI,DraI,HinfI,Hpy188I,RsaI,SsPI,TaqI All possible enzymes for lycopersicum and pimpinellifolium: BstZ17I,HinfI,Hpy188I
Accessions and product sizes Solanum pennellii LA716: 1200 Solanum lycopersicum LA925: 1200	Approximate temperature 55°C