SGN Marker C2_At2g21100

SGN-M7264
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

SynonymsNone
Locus associationsNone
Orthologs in this COSII group 
SpeciesCopiesSequence IDCDS/Edited sequencePeptide sequencePredicted introns
CoffeeSingle311244 (SGN)
126196 (CGN)
---
Lycopersicon CombinedSingleSGN-U227867---
Solanum tuberosumSingleSGN-U253328---
ArabidopsisSingleAt2g21100 on TAIR---
Mapped locations  
Map: Tomato-EXPEN 2000   
Map version
52 (location: 38316)
Chromosome
1
Position
165.00 cM
Confidence
I
Protocol
CAPS


Tomato-EXPEN 2000

PCR data   Exp. ID 39577
Forward primer (5'-3')
ACCTATTTATATGGGTGGCTCAAGC
Accessions and product sizes
Solanum lycopersicum TA209
350
Solanum pennellii TA56
350
Approximate temperature
55°C
Reverse primer (5'-3')
AACGATAGCCATCTCACGAACCG
Digested band sizes (using amplicon difference)
Solanum lycopersicum TA209
350
Solanum pennellii TA56
300
Mg+2 concentration
1.5mM
   Enzymes
  

Map: Arabidopsis COSII   
Map version
44 (location: 25679)
Chromosome
2
Position
9.06 MB
Confidence
uncalculated
Protocol
CAPS


Arabidopsis COSII
Other PCR data 
PCR data   Exp. ID 10621
Forward primer (5'-3')
ACCTATTTATATGGGTGGCTCAAGC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
AACGATAGCCATCTCACGAACCG
Enzymes
  
Accessions and product sizes
Solanum pennellii LA716: 400
Approximate temperature
55°C
  
PCR data   Exp. ID 10622
Forward primer (5'-3')
ACCTATTTATATGGGTGGCTCAAGC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
AACGATAGCCATCTCACGAACCG
Enzymes
  
Accessions and product sizes
Solanum lycopersicum LA925: 400
Approximate temperature
55°C
  
PCR data   Exp. ID 10623
Forward primer (5'-3')
ACCTATTTATATGGGTGGCTCAAGC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
AACGATAGCCATCTCACGAACCG
Enzymes
  
Accessions and product sizes
Solanum pimpinellifolium LA1589: 400
Approximate temperature
55°C
  
PCR data   Exp. ID 10624
Forward primer (5'-3')
ACCTATTTATATGGGTGGCTCAAGC
Digested band sizes (using Tsp509I)
Solanum lycopersicum LA925: 200
Solanum pennellii LA716: 250
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
AACGATAGCCATCTCACGAACCG
Enzymes
All possible enzymes for pennellii and and lycopersicum: BstYI,DpnII,Tsp509I
  
Accessions and product sizes
Solanum lycopersicum LA925: 400
Solanum pennellii LA716: 400
Approximate temperature
55°C
  
Unigene blast matches for primers 
Universal primers for Asterid species 
No additional PCR data found.
Other COSII sequence data 

Alignment of Arabidopsis CDS and edited Asterid unigenes, FASTA
Alignment of DNA and translated peptides from Arabidopsis CDS and edited Asterid unigenes, plain text
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, ClustalW
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, FASTA

Amplicon sequence alignment, FASTA
At2g21100F.cds.txt, plain text
Forward amplicon sequence for S. pimpinellifolium LA1589, AB1 chromatogram - [View]
Forward amplicon sequence for S. pimpinellifolium LA1589, plain text
Forward amplicon sequence for S. lycopersicum TA209, AB1 chromatogram - [View]
Forward amplicon sequence for S. lycopersicum TA209, plain text
Forward amplicon sequence for S. pennellii TA56, AB1 chromatogram - [View]
Forward amplicon sequence for S. pennellii TA56, plain text

Genomic location of C2_At2g21100  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D7264 marker 7264
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