Sol Genomics Network

SGN-M7313
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	300895 (SGN) 124974 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U198088	-	-	-
Lycopersicon Combined	Single	SGN-U218497	-	-	-
Solanum tuberosum	Single	SGN-U246712	-	-	-
Arabidopsis	Single	At2g26590 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39589

Forward primer (5'-3')

AGCCAGCAGCTGACAATGATGCAC

Accessions and product sizes

Solanum pennellii TA56

950

Solanum lycopersicum TA209

900

Approximate temperature

55°C

Reverse primer (5'-3')

TGGTAAAGGAGCTGAAGCTTCAGG

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

900

Solanum pennellii TA56

950

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10681
Forward primer (5'-3') AGCCAGCAGCTGACAATGATGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTAAAGGAGCTGAAGCTTCAGG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 950	Approximate temperature 55°C

PCR data Exp. ID 10682
Forward primer (5'-3') AGCCAGCAGCTGACAATGATGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTAAAGGAGCTGAAGCTTCAGG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 900	Approximate temperature 55°C

PCR data Exp. ID 10683
Forward primer (5'-3') AGCCAGCAGCTGACAATGATGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTAAAGGAGCTGAAGCTTCAGG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 900	Approximate temperature 55°C

PCR data Exp. ID 12999
Forward primer (5'-3') AGCCAGCAGCTGACAATGATGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTAAAGGAGCTGAAGCTTCAGG	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 900	Approximate temperature 55°C

PCR data Exp. ID 13000
Forward primer (5'-3') AGCCAGCAGCTGACAATGATGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTAAAGGAGCTGAAGCTTCAGG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 850	Approximate temperature 55°C

PCR data Exp. ID 13001
Forward primer (5'-3') AGCCAGCAGCTGACAATGATGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTAAAGGAGCTGAAGCTTCAGG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 850	Approximate temperature 55°C

PCR data Exp. ID 13002
Forward primer (5'-3') AGCCAGCAGCTGACAATGATGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTAAAGGAGCTGAAGCTTCAGG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 850	Approximate temperature 55°C

PCR data Exp. ID 13003
Forward primer (5'-3') AGCCAGCAGCTGACAATGATGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTAAAGGAGCTGAAGCTTCAGG	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 850	Approximate temperature 55°C

PCR data Exp. ID 13004
Forward primer (5'-3') AGCCAGCAGCTGACAATGATGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTAAAGGAGCTGAAGCTTCAGG	Enzymes
Accessions and product sizes Physalis TA1367: 850	Approximate temperature 55°C

PCR data Exp. ID 32929
Forward primer (5'-3') AGCCAGCAGCTGACAATGATGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTAAAGGAGCTGAAGCTTCAGG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 900	Approximate temperature 55°C

PCR data Exp. ID 32930
Forward primer (5'-3') AGCCAGCAGCTGACAATGATGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTAAAGGAGCTGAAGCTTCAGG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 850	Approximate temperature 55°C

PCR data Exp. ID 32931
Forward primer (5'-3') AGCCAGCAGCTGACAATGATGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTAAAGGAGCTGAAGCTTCAGG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 850	Approximate temperature 55°C

PCR data Exp. ID 32932
Forward primer (5'-3') AGCCAGCAGCTGACAATGATGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTAAAGGAGCTGAAGCTTCAGG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 850	Approximate temperature 55°C

PCR data Exp. ID 10684
Forward primer (5'-3') AGCCAGCAGCTGACAATGATGCAC	Digested band sizes (using amplicon difference) Solanum lycopersicum LA925: 900 Solanum pennellii LA716: 950	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTAAAGGAGCTGAAGCTTCAGG	Enzymes All possible enzymes for pennellii and and lycopersicum: amplicon difference
Accessions and product sizes Solanum lycopersicum LA925: 900 Solanum pennellii LA716: 950	Approximate temperature 55°C