Sol Genomics Network

SGN-M7318
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	310185 (SGN) 129859 (CGN)	-	-	-
Lycopersicon Combined	Single	SGN-U224701	-	-	-
Arabidopsis	Single	At2g27090 on TAIR	-	-	-

Map: Tomato - Kazusa and SolCAP markers mapped to genome
Map version 105 (location: 49228)
Chromosome 9
Position 0.75 MB
Confidence uncalculated
Protocol CAPS

Tomato - Kazusa and SolCAP markers mapped to genome

Kazusa F2-2000 genetic map

Tomato-EXPEN 2000

PCR data Exp. ID 39591

Forward primer (5'-3')

TCCGAAGTGATTATGATGCTAAGCG

Accessions and product sizes

Solanum pennellii TA56

500

Solanum lycopersicum TA209

500

Approximate temperature

55°C

Reverse primer (5'-3')

AGACAGAACAGGTGGTATAAATAGGTGG

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

500

Solanum pennellii TA56

200+300

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10691
Forward primer (5'-3') TCCGAAGTGATTATGATGCTAAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGACAGAACAGGTGGTATAAATAGGTGG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 700	Approximate temperature 55°C

PCR data Exp. ID 10692
Forward primer (5'-3') TCCGAAGTGATTATGATGCTAAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGACAGAACAGGTGGTATAAATAGGTGG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 700	Approximate temperature 55°C

PCR data Exp. ID 10693
Forward primer (5'-3') TCCGAAGTGATTATGATGCTAAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGACAGAACAGGTGGTATAAATAGGTGG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 650	Approximate temperature 55°C

PCR data Exp. ID 32244
Forward primer (5'-3') TCCGAAGTGATTATGATGCTAAGCG	Digested band sizes (using BstNI) Solanum pennellii LA716: 180+200+300 Solanum lycopersicum LA925: 200+500	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGACAGAACAGGTGGTATAAATAGGTGG	Enzymes All possible enzymes for pennellii and and lycopersicum: BstNI,ScrFI
Accessions and product sizes Solanum lycopersicum LA925: 700 Solanum pennellii LA716: 700	Approximate temperature 55°C