SGN Marker SSR63

SGN-M736

SynonymsNone
Locus associationsNone
SSR info 

Annealing temperatures: Low: 55 High: 40
Repeat motif: AT   Repeat number: 39
Repeat motif:    Repeat number:
Forward primer: CCACAAACAATTCCATCTCA
Reverse primer: GCTTCCGCCATACTGATACG
Predicted size: 250
Derivations 
This marker was derived from EST read SGN-E53346
Mapped locations  
Map: Sun1642 x LA1589   
Map version
85 (location: 43816)
Chromosome
8
Position
54.30 cM
Confidence
uncalculated
Protocol
SSR


Sun1642 x LA1589

PCR data   Exp. ID 44833
Forward primer (5'-3')
CCACAAACAATTCCATCTCA
Accessions and product sizes
Approximate temperature
55°C
Reverse primer (5'-3')
GCTTCCGCCATACTGATACG
Digested band sizes (using unknown enzyme)
Mg+2 concentration
Unknown
   Enzymes
  

Map: Integrated map: Yellow Stuffer X LA1589 and Sun1642 X LA1589   
Map version
79 (location: 43276)
Chromosome
8
Position
50.10 cM
Confidence
uncalculated
Protocol
SSR


Integrated map: Yellow Stuffer X LA1589 and Sun1642 X LA1589

PCR data   Exp. ID 44647
Forward primer (5'-3')
CCACAAACAATTCCATCTCA
Accessions and product sizes
Approximate temperature
55°C
Reverse primer (5'-3')
GCTTCCGCCATACTGATACG
Digested band sizes (using unknown enzyme)
Mg+2 concentration
Unknown
   Enzymes
  

Map: Tomato-LXCHM 2007   
Map version
68 (location: 43002)
Chromosome
8
Position
24.20 cM
Confidence
uncalculated
Protocol
SSR


Tomato-LXCHM 2007

PCR data   Exp. ID 44002
Forward primer (5'-3')
CCACAAACAATTCCATCTCA
Accessions and product sizes
Approximate temperature
55°C
Reverse primer (5'-3')
GCTTCCGCCATACTGATACG
Digested band sizes (using unknown enzyme)
Mg+2 concentration
Unknown
   Enzymes
  

Map: Tomato-EXPIMP 2008   
Map version
55 (location: 40769)
Chromosome
8
Position
61.00 cM
Confidence
uncalculated
Protocol
SSR


Tomato-EXPIMP 2008

Map: Tomato-EXPEN 2000   
Map version
52 (location: 39285)
Chromosome
8
Position
50.70 cM
Confidence
I
Protocol
SSR


Tomato-EXPEN 2000
Other PCR data 
PCR data   Exp. ID 31916
Forward primer (5'-3')
CCACAAACAATTCCATCTCA
 
 
Mg+2 concentration
Unknown
Reverse primer (5'-3')
GCTTCCGCCATACTGATACG
Enzymes
  
Accessions and product sizes
Approximate temperature
40°C
  
Overgo hybridization and physical mapping 
SSR63 was used as an overgo probe on plate 1 [well H1]

Plausible BAC Matches:   P315E12,   P275G05,   P176C05,   P315C12

 Non-Plausible BAC matches

P080C23,   P142J15,   P094M12,   P128E10,   P054F10,   P190C17,   P156M20

 Overgo Sequences

A sequence
ACTCCAAAATGGGCATGTCTTTGC
B sequence
TCCTTCCTTCCAACCAGCAAAGAC
AB sequence
ACTCCAAAATGGGCATGTCTTTGCTGGTTG
Marker sequence
CTCATTTCTCGTCCATATTTGACACAGATACAGATGAACCACAAACAATT
CCATCTCATGAGTAAGTACTTCTACAGTGAACTCAAAAACCATTTCAGTT
TTTCGCGTTAAGAGATGTTAATTCAACAACTACTAACAATATATATATAT
ATATATATATATATATATATATATATATATATATATATATATATATATAT
ATATATATATATATATCTTTTACTTCAATATCTACAGTCAGATATTCTGG
TTTGGAGACTTAAACTATCGTATCAGTATGGCGGAAGCTGAGGTACGGAA
GCTGGTTGCCAATAAGAAATGGGATGAACTACTCAACAGTGATCAATTGC
ACAAAGAACTCCAAAATGGGCATGTCTTTGCTGGTTGGAAGGAAGGATTG
GTTAACTTCGCACCAACTTACAAGTATGAATTCAACAGTGATAGATACAA
CGGAGAAATTACGAGAGAAGGGGAAAAGAAGAGATCACCAGCATGGTGTG
ATCGTATACTATGGTTAGGGAAAGGTATAAAACAGATGTTTTACACGCGC
GCAGAGTTAAAACTATCAGATCATATACCTGTAAGCTCAATGTTCTCAGT
ATAAGTGGAAATATTTGTCCACAGAAAACTACAGAGAGTTCTCAATGTTA
AAAGTGC
Genomic location of SSR63  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D736 marker 736
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