Sol Genomics Network

SGN-M7376
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Multiple	299657 (SGN) 122988 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U197760	-	-	-
Lycopersicon Combined	Single	SGN-U218453	-	-	-
Solanum tuberosum	Single	SGN-U248496	-	-	-
Arabidopsis	Single	At2g32600 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39604

Forward primer (5'-3')

TGAAGGGAATTACTTGGCTCACAC

Accessions and product sizes

Solanum lycopersicum TA209

1100

Solanum pennellii TA56

1100

Approximate temperature

55°C

Reverse primer (5'-3')

TGTTTTGTTTCCGGATCAAATTGC

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

1000

Solanum lycopersicum TA209

850

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10753
Forward primer (5'-3') TGAAGGGAATTACTTGGCTCACAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGTTTTGTTTCCGGATCAAATTGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1300	Approximate temperature 55°C

PCR data Exp. ID 10754
Forward primer (5'-3') TGAAGGGAATTACTTGGCTCACAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGTTTTGTTTCCGGATCAAATTGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1300	Approximate temperature 55°C

PCR data Exp. ID 10755
Forward primer (5'-3') TGAAGGGAATTACTTGGCTCACAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGTTTTGTTTCCGGATCAAATTGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1300	Approximate temperature 55°C

PCR data Exp. ID 32254
Forward primer (5'-3') TGAAGGGAATTACTTGGCTCACAC	Digested band sizes (using HinfI) Solanum pennellii LA716: 200+350+500 Solanum lycopersicum LA925: 500+550	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGTTTTGTTTCCGGATCAAATTGC	Enzymes All possible enzymes for pennellii and and lycopersicum: BglII,BstYI,HinfI,SspI,PflNI,DpnII,Hpy188I
Accessions and product sizes Solanum lycopersicum LA925: 1300 Solanum pennellii LA716: 1300	Approximate temperature 55°C