SGN Marker SSR75

SGN-M748

SynonymsNone
Locus associationsNone
SSR info 

Annealing temperatures: Low: 60 High: 40
Repeat motif: AAT   Repeat number: 9
Repeat motif:    Repeat number:
Forward primer: CCATCTATTATCTTCTCTCCAACAC
Reverse primer: GGTCCCAACTCGGTACACAC
Predicted size: 155
Derivations 
This marker was derived from EST read SGN-E157183
Mapped locations  
Map: Tomato-EXPEN 2000   
Map version
52 (location: 39296)
Chromosome
1
Position
53.50 cM
Confidence
I
Protocol
SSR


Tomato-EXPEN 2000
Other PCR data 
PCR data   Exp. ID 32052
Forward primer (5'-3')
CCATCTATTATCTTCTCTCCAACAC
 
 
Mg+2 concentration
Unknown
Reverse primer (5'-3')
GGTCCCAACTCGGTACACAC
Enzymes
  
Accessions and product sizes
Approximate temperature
40°C
  
Overgo hybridization and physical mapping 
SSR75 was used as an overgo probe on plate 9 [well A6]

Plausible BAC Matches:   None

 Overgo Sequences

A sequence
AACGTGGGAAAATGGGCAGTTAGC
B sequence
CCCTAACCCATGCATTGCTAACTG
AB sequence
AACGTGGGAAAATGGGCAGTTAGCAATGCA
Marker sequence
CTTATATCTACCCTTATTCCATCTATTATCTTCTCTCCAACACCAAAATT
TTGAGAGAATAATAATAATAATAATAATAATAATATATTGTTGTTTTGAT
TCATTCATCACCAAGAAGAACACCTCCCTCTTATTTACCTTCGATATGAA
TCAGTGTGTACCGAGTTGGGACCTCGATGACTCTACCGTTCCTAGAAAAA
ATCTTATACAAACTCAATCCAATTCATTAGCCGTTGATGTCCCCTCGTAT
ATACTCTCTATTACTCTTTCACAATAATTATTACTTTTATTTTATATTTA
CTGACTCAAAATTCTGAATTCGCAGATTAGATTATGAAGTAGCGGAGCTA
ACGTGGGAAAATGGGCAGTTAGCAATGCATGGGTTAGGGCCTCCACGGGC
TAATAATAAGCCCATATCGAGCTACGGTGGCACCCTTGAATCTATAGTGA
ACCAAGCCACCCGTTGCAACGACGACGTTCCTCTTCATCTCCACGGGAAG
TCCACCGTGGACAGGAACAAACAGAGTGGAGATGAGGTGGTTCCTTGGTT
CAACAACCACAACGCGGTTGCTTACGCTCCTCCGGCAACCGGTTTAGTCG
CAATGACTAAGGATGCGTTGGGTGCCGTGTTCGAGGAACACCTCAAATTC
GGACACCAACGATCCGTGCACGTGCCAGGGATTGACGGCTC
Genomic location of SSR75  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D748 marker 748
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