Sol Genomics Network

SGN-M7484
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	306554 (SGN) 131611 (CGN)	-	-	-
Lycopersicon Combined	Single	SGN-U221908	-	-	-
Arabidopsis	Single	At2g41680 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39632

Forward primer (5'-3')

TCTGTGGAAGGTGTATTTGCAGC

Accessions and product sizes

Solanum pennellii TA56

880

Solanum lycopersicum TA209

880

Approximate temperature

55°C

Reverse primer (5'-3')

AGTGATGGCTTGCCTCCATTC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

650

Solanum pennellii TA56

500

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10844
Forward primer (5'-3') TCTGTGGAAGGTGTATTTGCAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTGATGGCTTGCCTCCATTC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 600	Approximate temperature 55°C

PCR data Exp. ID 10845
Forward primer (5'-3') TCTGTGGAAGGTGTATTTGCAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTGATGGCTTGCCTCCATTC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 600	Approximate temperature 55°C

PCR data Exp. ID 10846
Forward primer (5'-3') TCTGTGGAAGGTGTATTTGCAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTGATGGCTTGCCTCCATTC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 600	Approximate temperature 55°C

PCR data Exp. ID 10847
Forward primer (5'-3') TCTGTGGAAGGTGTATTTGCAGC	Digested band sizes (using ApoI) Solanum pennellii LA716: 150 Solanum lycopersicum LA925: 290	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTGATGGCTTGCCTCCATTC	Enzymes All possible enzymes for pennellii and and lycopersicum: ApoI
Accessions and product sizes Solanum lycopersicum LA925: 600 Solanum pennellii LA716: 600	Approximate temperature 55°C