Sol Genomics Network

SGN-M7508
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	308049 (SGN) 125690 (CGN)	-	-	-
Capsicum annuum	Multiple	SGN-U196795	-	-	-
Lycopersicon Combined	Single	SGN-U224436	-	-	-
Solanum tuberosum	Single	SGN-U248233	-	-	-
Arabidopsis	Single	At2g43770 on TAIR	-	-	-

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46645)
Chromosome 6
Position 23.40 cM
Confidence F(LOD3)
Protocol CAPS

Tobacco N. tomentosiformis

Tomato-EXPEN 2000

PCR data Exp. ID 39639

Forward primer (5'-3')

AATGGAGTTGTCTGTTGTGCTTCC

Accessions and product sizes

Approximate temperature

Unknown

Reverse primer (5'-3')

TGGCGCATATCCCAAAGTTTAGC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10860
Forward primer (5'-3') AATGGAGTTGTCTGTTGTGCTTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCGCATATCCCAAAGTTTAGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 450	Approximate temperature 55°C

PCR data Exp. ID 10861
Forward primer (5'-3') AATGGAGTTGTCTGTTGTGCTTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCGCATATCCCAAAGTTTAGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 450	Approximate temperature 55°C

PCR data Exp. ID 10862
Forward primer (5'-3') AATGGAGTTGTCTGTTGTGCTTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCGCATATCCCAAAGTTTAGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 450	Approximate temperature 55°C

PCR data Exp. ID 10863
Forward primer (5'-3') AATGGAGTTGTCTGTTGTGCTTCC	Digested band sizes (using MspI) Solanum pennellii LA716: 330 Solanum lycopersicum LA925: 450	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGCGCATATCCCAAAGTTTAGC	Enzymes All possible enzymes for pennellii and and lycopersicum: MspI
Accessions and product sizes Solanum pennellii LA716: 450 Solanum lycopersicum LA925: 450	Approximate temperature 55°C