Sol Genomics Network

SGN-M7924
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	307459 (SGN) 128488 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U201540	-	-	-
Lycopersicon Combined	Single	SGN-U216273	-	-	-
Solanum tuberosum	Multiple	SGN-U245153	-	-	-
Arabidopsis	Single	At3g44880 on TAIR	-	-	-

Map: Tomato - Kazusa and SolCAP markers mapped to genome
Map version 105 (location: 49250)
Chromosome 11
Position 49.29 MB
Confidence uncalculated
Protocol CAPS

Tomato - Kazusa and SolCAP markers mapped to genome

Kazusa F2-2000 genetic map

Tomato-EXPEN 2000

PCR data Exp. ID 39748

Forward primer (5'-3')

ACAAGATTCGTCGTCGAAATTCTC

Accessions and product sizes

Solanum pennellii TA56

280

Solanum lycopersicum TA209

280

Approximate temperature

55°C

Reverse primer (5'-3')

ACCACATCCATTAAATGACCATCC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

280

Solanum pennellii TA56

200

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11179
Forward primer (5'-3') ACAAGATTCGTCGTCGAAATTCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCACATCCATTAAATGACCATCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 320	Approximate temperature 55°C

PCR data Exp. ID 11180
Forward primer (5'-3') ACAAGATTCGTCGTCGAAATTCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCACATCCATTAAATGACCATCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 320	Approximate temperature 55°C

PCR data Exp. ID 11181
Forward primer (5'-3') ACAAGATTCGTCGTCGAAATTCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCACATCCATTAAATGACCATCC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 320	Approximate temperature 55°C

PCR data Exp. ID 11182
Forward primer (5'-3') ACAAGATTCGTCGTCGAAATTCTC	Digested band sizes (using DraI) Solanum lycopersicum LA925: 260 Solanum pennellii LA716: 320	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCACATCCATTAAATGACCATCC	Enzymes All possible enzymes for pennellii and and lycopersicum: DraI,HinfI,TaqI,Tsp509I All possible enzymes for lycopersicum and pimpinellifolium: MseI/Tru1I/Tru9I
Accessions and product sizes Solanum lycopersicum LA925: 320 Solanum pennellii LA716: 320	Approximate temperature 55°C