Sol Genomics Network

SGN-M7944
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	303934 (SGN) 120673 (CGN)	-	-	-
Capsicum annuum	Multiple	SGN-U200272	-	-	-
Lycopersicon Combined	Multiple	SGN-U234613	-	-	-
Solanum tuberosum	Single	SGN-U258835	-	-	-
Arabidopsis	Single	At3g47990 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39754

Forward primer (5'-3')

AGAGAAGCAGTGGAGGCACTCATTC

Accessions and product sizes

Solanum pennellii TA56

850

Solanum lycopersicum TA209

750

Approximate temperature

55°C

Reverse primer (5'-3')

AGAAAACCTTGCAACCTCAGCAG

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

750

Solanum pennellii TA56

850

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11199
Forward primer (5'-3') AGAGAAGCAGTGGAGGCACTCATTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGAAAACCTTGCAACCTCAGCAG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1100	Approximate temperature 55°C

PCR data Exp. ID 11200
Forward primer (5'-3') AGAGAAGCAGTGGAGGCACTCATTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGAAAACCTTGCAACCTCAGCAG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1100	Approximate temperature 55°C

PCR data Exp. ID 11201
Forward primer (5'-3') AGAGAAGCAGTGGAGGCACTCATTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGAAAACCTTGCAACCTCAGCAG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1100	Approximate temperature 55°C

PCR data Exp. ID 11202
Forward primer (5'-3') AGAGAAGCAGTGGAGGCACTCATTC	Digested band sizes (using HaeIII) Solanum lycopersicum LA925: 1100 Solanum pennellii LA716: 780+320	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGAAAACCTTGCAACCTCAGCAG	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI,ApoI,BsaHI,BslI,EcoNI,HaeIII,HinfI,MspI,SsPI,TspRI All possible enzymes for lycopersicum and pimpinellifolium: AhlI/BcuI/SpeI,BfaI/FspBI/MaeI/XspI
Accessions and product sizes Solanum pennellii LA716: 1100 Solanum lycopersicum LA925: 1100	Approximate temperature 55°C