Sol Genomics Network

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SGN Marker C2_At3g54470

SGN-M8028
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	304071 (SGN) 121896 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U197543	-	-	-
Lycopersicon Combined	Single	SGN-U217894	-	-	-
Solanum tuberosum	Multiple	SGN-U248357	-	-	-
Arabidopsis	Single	At3g54470 on TAIR	-	-	-

Mapped locations

Map: Tomato-EXPEN 2000
Map version 52 (location: 38565)
Chromosome 11
Position 72.00 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39766

Forward primer (5'-3')

TCCTGACTTTGGTTCTAAGCTTAGATCG

Accessions and product sizes

Solanum pennellii TA56

1100

Solanum lycopersicum TA209

1200

Approximate temperature

55°C

Reverse primer (5'-3')

TCAAATATTAAGAAGTTGTGCTTGTCTGC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

1200

Solanum pennellii TA56

1100

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 26463)
Chromosome 3
Position 20.18 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11240
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 700	Approximate temperature 55°C

PCR data Exp. ID 11241
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 700	Approximate temperature 55°C

PCR data Exp. ID 11242
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 700	Approximate temperature 55°C

PCR data Exp. ID 13312
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 650	Approximate temperature 55°C

PCR data Exp. ID 13313
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 750	Approximate temperature 55°C

PCR data Exp. ID 13314
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 700	Approximate temperature 55°C

PCR data Exp. ID 13315
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 550	Approximate temperature 55°C

PCR data Exp. ID 13316
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 550+650	Approximate temperature 55°C

PCR data Exp. ID 13317
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 550	Approximate temperature 55°C

PCR data Exp. ID 13318
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Physalis TA1367: 700	Approximate temperature 55°C

PCR data Exp. ID 13319
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 700	Approximate temperature 55°C

PCR data Exp. ID 13320
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 550+750	Approximate temperature 55°C

PCR data Exp. ID 13321
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 550+750	Approximate temperature 55°C

PCR data Exp. ID 13322
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Coffea canephora BP409: 580	Approximate temperature 55°C

PCR data Exp. ID 33057
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 550	Approximate temperature 55°C

PCR data Exp. ID 33058
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 550+650	Approximate temperature 55°C

PCR data Exp. ID 33059
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 550	Approximate temperature 55°C

PCR data Exp. ID 11243
Forward primer (5'-3') TCCTGACTTTGGTTCTAAGCTTAGATCG	Digested band sizes (using RsaI) Solanum pennellii LA716: 700 Solanum lycopersicum LA925: 450+225	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAAATATTAAGAAGTTGTGCTTGTCTGC	Enzymes All possible enzymes for pennellii and and lycopersicum: MspI,RsaI,DraI,TaqI,RV,HaeIII,HinfI,DpnII,Kpni
Accessions and product sizes Solanum pennellii LA716: 700 Solanum lycopersicum LA925: 700	Approximate temperature 55°C