Sol Genomics Network

SGN Marker C2_At4g03210

SGN-M8182
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	300349 (SGN) 120895 (CGN)	-	-	-
Capsicum annuum	Multiple	SGN-U197350	-	-	-
Lycopersicon Combined	Single	SGN-U217823	-	-	-
Arabidopsis	Single	At4g03210 on TAIR	-	-	-

Mapped locations

Map: Eggplant-RAD 2012
Map version 174 (location: 132189)
Chromosome 7
Position 34.94 cM
Confidence uncalculated
Protocol CAPS

Eggplant-RAD 2012

Map: Pepper-COSII
Map version 58 (location: 41462)
Chromosome 7
Position 67.90 cM
Confidence F(LOD3)
Protocol CAPS

Pepper-COSII

PCR data Exp. ID 43438

Forward primer (5'-3')

TGTTGAAGGTGACTCTGCTGGAACTG

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

TCTCTGTTCTCTGTTACCAACACCATTG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38615)
Chromosome 7
Position 44.70 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39804

Forward primer (5'-3')

TGTTGAAGGTGACTCTGCTGGAACTG

Accessions and product sizes

Solanum pennellii TA56

2100

Solanum lycopersicum TA209

2100

Approximate temperature

55°C

Reverse primer (5'-3')

TCTCTGTTCTCTGTTACCAACACCATTG

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

2100

Solanum lycopersicum TA209

1000+1100

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 26619)
Chromosome 4
Position 1.42 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11347
Forward primer (5'-3') TGTTGAAGGTGACTCTGCTGGAACTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTCTGTTCTCTGTTACCAACACCATTG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 600	Approximate temperature 55°C

PCR data Exp. ID 11348
Forward primer (5'-3') TGTTGAAGGTGACTCTGCTGGAACTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTCTGTTCTCTGTTACCAACACCATTG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 600	Approximate temperature 55°C

PCR data Exp. ID 11349
Forward primer (5'-3') TGTTGAAGGTGACTCTGCTGGAACTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTCTGTTCTCTGTTACCAACACCATTG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 600	Approximate temperature 55°C

PCR data Exp. ID 11350
Forward primer (5'-3') TGTTGAAGGTGACTCTGCTGGAACTG	Digested band sizes (using DraI) Solanum lycopersicum LA925: 400 Solanum pennellii LA716: 600	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTCTGTTCTCTGTTACCAACACCATTG	Enzymes All possible enzymes for pennellii and and lycopersicum: DraI, HinfI, DpnII
Accessions and product sizes Solanum pennellii LA716: 600 Solanum lycopersicum LA925: 600	Approximate temperature 55°C