Sol Genomics Network

SGN Marker C2_At4g12230

SGN-M8227
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Capsicum annuum	Single	SGN-U204269	-	-	-
Lycopersicon Combined	Single	SGN-U225001	-	-	-
Solanum tuberosum	Multiple	SGN-U250460	-	-	-
Arabidopsis	Single	At4g12230 on TAIR	-	-	-

Mapped locations

Map: Pepper-COSII
Map version 58 (location: 41469)
Chromosome 1
Position 40.70 cM
Confidence F(LOD3)
Protocol dCAPS

Pepper-COSII

PCR data Exp. ID 43445

Forward primer (5'-3')

AAGTGATCTAATATCTTTTTGTATATATAG

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

TCCACTTAGCACATGCCGTTTCAG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38633)
Chromosome 8
Position 63.80 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39819

Forward primer (5'-3')

TCTCAGCTTCAATGCCATGGGCTC

Accessions and product sizes

Solanum lycopersicum TA209

350

Solanum pennellii TA56

350

Approximate temperature

55°C

Reverse primer (5'-3')

TCCACTTAGCACATGCCGTTTCAG

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

80+100

Solanum lycopersicum TA209

100+150

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 26668)
Chromosome 4
Position 7.29 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11397
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 350	Approximate temperature 55°C

PCR data Exp. ID 11398
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 350	Approximate temperature 55°C

PCR data Exp. ID 11399
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 350	Approximate temperature 55°C

PCR data Exp. ID 13389
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 350	Approximate temperature 55°C

PCR data Exp. ID 13390
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Solanum melongena TA2986: 350	Approximate temperature 55°C

PCR data Exp. ID 13391
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 300	Approximate temperature 55°C

PCR data Exp. ID 13392
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1100	Approximate temperature 55°C

PCR data Exp. ID 13393
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 350	Approximate temperature 55°C

PCR data Exp. ID 13394
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 350	Approximate temperature 55°C

PCR data Exp. ID 13395
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 350	Approximate temperature 55°C

PCR data Exp. ID 13396
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 350	Approximate temperature 55°C

PCR data Exp. ID 13397
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Physalis TA1367: 350	Approximate temperature 55°C

PCR data Exp. ID 13398
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 350	Approximate temperature 55°C

PCR data Exp. ID 13399
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 350	Approximate temperature 55°C

PCR data Exp. ID 13400
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 350	Approximate temperature 55°C

PCR data Exp. ID 33086
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1100	Approximate temperature 55°C

PCR data Exp. ID 33087
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 350	Approximate temperature 55°C

PCR data Exp. ID 33088
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 350	Approximate temperature 55°C

PCR data Exp. ID 33089
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 350	Approximate temperature 55°C

PCR data Exp. ID 11400
Forward primer (5'-3') TCTCAGCTTCAATGCCATGGGCTC	Digested band sizes (using AluI) Solanum lycopersicum LA925: 150+100 Solanum pennellii LA716: 100+80	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCACTTAGCACATGCCGTTTCAG	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI
Accessions and product sizes Solanum pennellii LA716: 350 Solanum lycopersicum LA925: 350	Approximate temperature 55°C