SGN Marker C2_At4g15530

SGN-M8253
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

SynonymsNone
Locus associationsNone
Orthologs in this COSII group 
SpeciesCopiesSequence IDCDS/Edited sequencePeptide sequencePredicted introns
CoffeeSingle301102 (SGN)
122715 (CGN)
---
Lycopersicon CombinedMultipleSGN-U215938---
Solanum tuberosumSingleSGN-U254553---
ArabidopsisSingleAt4g15530 on TAIR---
Mapped locations  
Map: Tomato-EXPEN 2000   
Map version
52 (location: 38642)
Chromosome
1
Position
42.60 cM
Confidence
I(LOD2)
Protocol
CAPS


Tomato-EXPEN 2000

PCR data   Exp. ID 39826
Forward primer (5'-3')
TGTAAAGATGGTGGAGCCACAGC
Accessions and product sizes
Solanum pennellii TA56
200
Solanum lycopersicum TA209
200
Approximate temperature
55°C
Reverse primer (5'-3')
ATCCACGGGCTACAACAGCTGC
Digested band sizes (using unknown enzyme)
Mg+2 concentration
1.5mM
   Enzymes
  

Map: Arabidopsis COSII   
Map version
44 (location: 26695)
Chromosome
4
Position
8.87 MB
Confidence
uncalculated
Protocol
CAPS


Arabidopsis COSII
Other PCR data 
PCR data   Exp. ID 11413
Forward primer (5'-3')
TGTAAAGATGGTGGAGCCACAGC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
ATCCACGGGCTACAACAGCTGC
Enzymes
  
Accessions and product sizes
Solanum pennellii LA716: 700
Approximate temperature
55°C
  
PCR data   Exp. ID 11414
Forward primer (5'-3')
TGTAAAGATGGTGGAGCCACAGC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
ATCCACGGGCTACAACAGCTGC
Enzymes
  
Accessions and product sizes
Solanum lycopersicum LA925: 700
Approximate temperature
55°C
  
PCR data   Exp. ID 11415
Forward primer (5'-3')
TGTAAAGATGGTGGAGCCACAGC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
ATCCACGGGCTACAACAGCTGC
Enzymes
  
Accessions and product sizes
Solanum pimpinellifolium LA1589: 700
Approximate temperature
55°C
  
PCR data   Exp. ID 11416
Forward primer (5'-3')
TGTAAAGATGGTGGAGCCACAGC
Digested band sizes (using ScaI)
Solanum lycopersicum LA925: 700
Solanum pennellii LA716: 650
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
ATCCACGGGCTACAACAGCTGC
Enzymes
All possible enzymes for pennellii and and lycopersicum: AseI,CfoI/HhaI,Hpy188I,NcoI,NsiI,PflMI,RsaI,ScaI,StyI,Tsp509I
All possible enzymes for lycopersicum and pimpinellifolium: AseI,EcoRV,NcoI,NsiI,RsaI,ScaI,StyI,Tsp509I,TspRI
  
Accessions and product sizes
Solanum lycopersicum LA925: 700
Solanum pennellii LA716: 700
Approximate temperature
55°C
  
Unigene blast matches for primers 
Universal primers for Asterid species 
No additional PCR data found.
Other COSII sequence data 

Alignment of Arabidopsis CDS and edited Asterid unigenes, FASTA
Alignment of DNA and translated peptides from Arabidopsis CDS and edited Asterid unigenes, plain text
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, ClustalW
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, FASTA

Amplicon sequence alignment, FASTA
At4g15530F.cds.txt, plain text
Forward amplicon sequence for S. pimpinellifolium LA1589, AB1 chromatogram - [View]
Forward amplicon sequence for S. pimpinellifolium LA1589, PDF chromatogram
Forward amplicon sequence for S. pimpinellifolium LA1589, plain text
Forward amplicon sequence for S. lycopersicum TA209, AB1 chromatogram - [View]
Forward amplicon sequence for S. lycopersicum TA209, PDF chromatogram
Forward amplicon sequence for S. lycopersicum TA209, plain text
Forward amplicon sequence for S. pennellii TA56, AB1 chromatogram - [View]
Forward amplicon sequence for S. pennellii TA56, PDF chromatogram
Forward amplicon sequence for S. pennellii TA56, plain text

Genomic location of C2_At4g15530  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D8253 marker 8253
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