SGN Marker C2_At4g22670

SGN-M8332
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

SynonymsNone
Locus associationsNone
Orthologs in this COSII group 
SpeciesCopiesSequence IDCDS/Edited sequencePeptide sequencePredicted introns
CoffeeMultiple299660 (SGN)
119680 (CGN)		---
Capsicum annuumSingleSGN-U196448---
Lycopersicon CombinedSingleSGN-U214772---
Solanum tuberosumMultipleSGN-U244170---
ArabidopsisSingleAt4g22670 on TAIR---
Mapped locations  
Map: Tomato-EXPEN 2000   
Map version
52 (location: 38670)
Chromosome
8
Position
64.00 cM
Confidence
I
Protocol
CAPS


Tomato-EXPEN 2000

PCR data   Exp. ID 39847
Forward primer (5'-3')
TGGGAGGCAGCTGCTAAGGATCTTC
Accessions and product sizes
Solanum lycopersicum TA209
250
Solanum pennellii TA56
250
Approximate temperature
53°C
Reverse primer (5'-3')
TCTTTCTATCTTCTCGTTCTTTGCG
Digested band sizes (using amplicon difference)
Solanum lycopersicum TA209
120
Solanum pennellii TA56
180
Mg+2 concentration
1.5mM
   Enzymes
  

Map: Arabidopsis COSII   
Map version
44 (location: 26773)
Chromosome
4
Position
11.92 MB
Confidence
uncalculated
Protocol
CAPS


Arabidopsis COSII
Other PCR data 
PCR data   Exp. ID 11464
Forward primer (5'-3')
TGGGAGGCAGCTGCTAAGGATCTTC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
TCTTTCTATCTTCTCGTTCTTTGCG
Enzymes
  
Accessions and product sizes
Solanum pennellii LA716: 350
 Approximate temperature
55°C
  
PCR data   Exp. ID 11465
Forward primer (5'-3')
TGGGAGGCAGCTGCTAAGGATCTTC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
TCTTTCTATCTTCTCGTTCTTTGCG
Enzymes
  
Accessions and product sizes
Solanum lycopersicum LA925: 350
 Approximate temperature
55°C
  
PCR data   Exp. ID 11466
Forward primer (5'-3')
TGGGAGGCAGCTGCTAAGGATCTTC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
TCTTTCTATCTTCTCGTTCTTTGCG
Enzymes
  
Accessions and product sizes
Solanum pimpinellifolium LA1589: 350
 Approximate temperature
55°C
  
PCR data   Exp. ID 11467
Forward primer (5'-3')
TGGGAGGCAGCTGCTAAGGATCTTC
Digested band sizes (using TaqI)
Solanum lycopersicum LA925: 290
Solanum pennellii LA716: 350
 Mg+2 concentration
1.5mM
Reverse primer (5'-3')
TCTTTCTATCTTCTCGTTCTTTGCG
Enzymes
All possible enzymes for pennellii and and lycopersicum: TaqI
   
Accessions and product sizes
Solanum pennellii LA716: 350
Solanum lycopersicum LA925: 350
 Approximate temperature
55°C
  
Unigene blast matches for primers 
SGN-U214772
SGN-U269474
Universal primers for Asterid species 
No additional PCR data found.
Other COSII sequence data 
BLASTX result of original unigene sequences against Arabidopsis protein database
Alignment of Arabidopsis CDS and edited Asterid unigenes, FASTA
Alignment of DNA and translated peptides from Arabidopsis CDS and edited Asterid unigenes, plain text
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, ClustalW
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, FASTA
Input file for PAUP, NEXUS format
Phylogenetic tree
Phylogenetic tree   |   [View]
Forward amplicon sequence for S. betaceum 6002061 plant #4, AB1 chromatogram - [View]
Forward amplicon sequence for S. betaceum 6002061 plant #4, plain text
Forward amplicon sequence for S. uniloba 6002078 plant #6, AB1 chromatogram - [View]
Forward amplicon sequence for S. uniloba 6002078 plant #6, plain text
BLASTX result of original unigene sequences against Arabidopsis protein database
Alignment of Arabidopsis CDS and edited Asterid unigenes, FASTA
Alignment of DNA and translated peptides from Arabidopsis CDS and edited Asterid unigenes, plain text
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, ClustalW
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, FASTA
Input file for PAUP, NEXUS format
Phylogenetic tree
Phylogenetic tree   |   [View]
Forward amplicon sequence for S. betaceum 6002061 plant #4, AB1 chromatogram - [View]
Forward amplicon sequence for S. betaceum 6002061 plant #4, plain text
Forward amplicon sequence for S. uniloba 6002078 plant #6, AB1 chromatogram - [View]
Forward amplicon sequence for S. uniloba 6002078 plant #6, plain text
Related Markers  

Related Genotyped Markers: These markers have been genotyped and share a name with this marker
Genomic location of C2_At4g22670  
 %2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D8332 marker 8332
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