Sol Genomics Network

SGN-M8353
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Capsicum annuum	Single	SGN-U201078	-	-	-
Lycopersicon Combined	Single	SGN-U217793	-	-	-
Solanum tuberosum	Single	SGN-U246253	-	-	-
Arabidopsis	Single	At4g24620 on TAIR	-	-	-

Arabidopsis COSII

PCR data Exp. ID 41593

Forward primer (5'-3')

TACTTGCTTTGTGTTGGTACTGGGC

Accessions and product sizes

Solanum lycopersicum TA209

850

Solanum pennellii TA56

850

Approximate temperature

55°C

Reverse primer (5'-3')

ATGACAAGCTGCTGTAAATACCTACTG

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

350+500

Solanum pennellii TA56

360

Mg⁺² concentration

1.5mM

Enzymes

Other PCR data

PCR data Exp. ID 11480
Forward primer (5'-3') TACTTGCTTTGTGTTGGTACTGGGC		Mg⁺² concentration 3mM
Reverse primer (5'-3') ATGACAAGCTGCTGTAAATACCTACTG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 230	Approximate temperature 53°C

PCR data Exp. ID 11481
Forward primer (5'-3') TACTTGCTTTGTGTTGGTACTGGGC		Mg⁺² concentration 3mM
Reverse primer (5'-3') ATGACAAGCTGCTGTAAATACCTACTG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 230	Approximate temperature 53°C

PCR data Exp. ID 11482
Forward primer (5'-3') TACTTGCTTTGTGTTGGTACTGGGC		Mg⁺² concentration 3mM
Reverse primer (5'-3') ATGACAAGCTGCTGTAAATACCTACTG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 230	Approximate temperature 53°C