Sol Genomics Network

SGN-M8354
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	300628 (SGN) 120170 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U197947	-	-	-
Lycopersicon Combined	Single	SGN-U219136	-	-	-
Solanum tuberosum	Multiple	SGN-U244336	-	-	-
Arabidopsis	Single	At4g24690 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39849

Forward primer (5'-3')

TTGCCTTCAGGGGAGAAGTTTGG

Accessions and product sizes

Solanum lycopersicum TA209

150

Solanum pennellii TA56

150

Approximate temperature

53°C

Reverse primer (5'-3')

TCAACTAATTCCATGGCAGGGTTTG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11483
Forward primer (5'-3') TTGCCTTCAGGGGAGAAGTTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAACTAATTCCATGGCAGGGTTTG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 350	Approximate temperature 53°C

PCR data Exp. ID 11484
Forward primer (5'-3') TTGCCTTCAGGGGAGAAGTTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAACTAATTCCATGGCAGGGTTTG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 350	Approximate temperature 53°C

PCR data Exp. ID 11485
Forward primer (5'-3') TTGCCTTCAGGGGAGAAGTTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAACTAATTCCATGGCAGGGTTTG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 350	Approximate temperature 53°C

PCR data Exp. ID 13452
Forward primer (5'-3') TTGCCTTCAGGGGAGAAGTTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAACTAATTCCATGGCAGGGTTTG	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 350	Approximate temperature 55°C

PCR data Exp. ID 13453
Forward primer (5'-3') TTGCCTTCAGGGGAGAAGTTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAACTAATTCCATGGCAGGGTTTG	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 350	Approximate temperature 55°C

PCR data Exp. ID 13454
Forward primer (5'-3') TTGCCTTCAGGGGAGAAGTTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAACTAATTCCATGGCAGGGTTTG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 350	Approximate temperature 55°C

PCR data Exp. ID 13455
Forward primer (5'-3') TTGCCTTCAGGGGAGAAGTTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAACTAATTCCATGGCAGGGTTTG	Enzymes
Accessions and product sizes Physalis TA1367: 350	Approximate temperature 55°C

PCR data Exp. ID 13456
Forward primer (5'-3') TTGCCTTCAGGGGAGAAGTTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAACTAATTCCATGGCAGGGTTTG	Enzymes
Accessions and product sizes Petunia axillaris TA3309: Multiple	Approximate temperature 55°C

PCR data Exp. ID 13457
Forward primer (5'-3') TTGCCTTCAGGGGAGAAGTTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAACTAATTCCATGGCAGGGTTTG	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: Multiple	Approximate temperature 55°C

PCR data Exp. ID 13458
Forward primer (5'-3') TTGCCTTCAGGGGAGAAGTTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAACTAATTCCATGGCAGGGTTTG	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: Multiple	Approximate temperature 55°C

PCR data Exp. ID 32644
Forward primer (5'-3') TTGCCTTCAGGGGAGAAGTTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAACTAATTCCATGGCAGGGTTTG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 350	Approximate temperature 55°C

PCR data Exp. ID 33107
Forward primer (5'-3') TTGCCTTCAGGGGAGAAGTTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAACTAATTCCATGGCAGGGTTTG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 350	Approximate temperature 55°C

PCR data Exp. ID 33108
Forward primer (5'-3') TTGCCTTCAGGGGAGAAGTTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAACTAATTCCATGGCAGGGTTTG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 350	Approximate temperature 55°C

PCR data Exp. ID 33109
Forward primer (5'-3') TTGCCTTCAGGGGAGAAGTTTGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAACTAATTCCATGGCAGGGTTTG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 350	Approximate temperature 55°C

PCR data Exp. ID 11486
Forward primer (5'-3') TTGCCTTCAGGGGAGAAGTTTGG	Digested band sizes (using DraI) Solanum pennellii LA716: 350 Solanum lycopersicum LA925: 330	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAACTAATTCCATGGCAGGGTTTG	Enzymes All possible enzymes for pennellii and and lycopersicum: RsaI, DraI, TaqI, EcoRv, HaeIII
Accessions and product sizes Solanum pennellii LA716: 350 Solanum lycopersicum LA925: 350	Approximate temperature 53°C