Sol Genomics Network

SGN Marker C2_At4g24830

SGN-M8358
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	302935 (SGN) 123764 (CGN)	-	-	-
Lycopersicon Combined	Multiple	SGN-U214048	-	-	-
Solanum tuberosum	Single	SGN-U266353	-	-	-
Arabidopsis	Single	At4g24830 on TAIR	-	-	-

Mapped locations

Map: Eggplant-RAD 2012
Map version 174 (location: 132190)
Chromosome 10
Position 67.49 cM
Confidence uncalculated
Protocol CAPS

Eggplant-RAD 2012

Map: Eggplant-COSII
Map version 63 (location: 42243)
Chromosome 10
Position 69.80 cM
Confidence I
Protocol CAPS

Eggplant-COSII

PCR data Exp. ID 43810

Forward primer (5'-3')

TGATGTTGCCAGAGAGGTTGGAGC

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

TCAGAGCAAAGAATGTCAGCTCAA

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38675)
Chromosome 5
Position 51.00 cM
Confidence I(LOD2)
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39852

Forward primer (5'-3')

ATACTTGCTTGGGACATCAATGGC

Accessions and product sizes

Solanum lycopersicum TA209

300

Solanum pennellii TA56

300

Approximate temperature

55°C

Reverse primer (5'-3')

TCCAACCTCTCTGGCAACATCAACC

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

280

Solanum lycopersicum TA209

300

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 26799)
Chromosome 4
Position 12.79 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11491
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1350	Approximate temperature 55°C

PCR data Exp. ID 11492
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1350	Approximate temperature 55°C

PCR data Exp. ID 11493
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1350	Approximate temperature 55°C

PCR data Exp. ID 13459
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 1500	Approximate temperature 55°C

PCR data Exp. ID 13460
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Solanum melongena TA2986: Multiple	Approximate temperature 55°C

PCR data Exp. ID 13461
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: Multiple	Approximate temperature 55°C

PCR data Exp. ID 13462
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: Multiple	Approximate temperature 55°C

PCR data Exp. ID 13463
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 500	Approximate temperature 55°C

PCR data Exp. ID 13464
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 500	Approximate temperature 55°C

PCR data Exp. ID 13465
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 500	Approximate temperature 55°C

PCR data Exp. ID 13466
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Physalis TA1367: 1500	Approximate temperature 55°C

PCR data Exp. ID 13467
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 900	Approximate temperature 55°C

PCR data Exp. ID 13468
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 850+500	Approximate temperature 55°C

PCR data Exp. ID 13469
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 850	Approximate temperature 55°C

PCR data Exp. ID 13470
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 900	Approximate temperature 55°C

PCR data Exp. ID 13471
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Coffea canephora BP409: 550	Approximate temperature 55°C

PCR data Exp. ID 33110
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: Multiple	Approximate temperature 55°C

PCR data Exp. ID 33111
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 500	Approximate temperature 55°C

PCR data Exp. ID 33112
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 500	Approximate temperature 55°C

PCR data Exp. ID 33113
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 500	Approximate temperature 55°C

PCR data Exp. ID 11494
Forward primer (5'-3') ATACTTGCTTGGGACATCAATGGC	Digested band sizes (using MspI) Solanum lycopersicum LA925: 1250 Solanum pennellii LA716: 1350	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAACCTCTCTGGCAACATCAACC	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI,MspI,RsaI,DraI,TaqI,RV,HaeIII,DpnII,KpnI All possible enzymes for lycopersicum and pimpinellifolium: MspI
Accessions and product sizes Solanum lycopersicum LA925: 1350 Solanum pennellii LA716: 1350	Approximate temperature 55°C