Sol Genomics Network

SGN Marker C2_At4g26750

SGN-M8374
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	302171 (SGN) 120413 (CGN)	-	-	-
Lycopersicon Combined	Multiple	SGN-U222974	-	-	-
Solanum tuberosum	Single	SGN-U247884	-	-	-
Arabidopsis	Single	At4g26750 on TAIR	-	-	-

Mapped locations

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46726)
Chromosome 8
Position 12.00 cM
Confidence CF(LOD3)
Protocol CAPS

Tobacco N. tomentosiformis

Map: Tobacco N. acuminata
Map version 89 (location: 46509)
Chromosome 8
Position 6.40 cM
Confidence F(LOD3)
Protocol CAPS

Tobacco N. acuminata

Map: Pepper-COSII
Map version 58 (location: 41485)
Chromosome 7
Position 110.40 cM
Confidence I
Protocol CAPS

Pepper-COSII

PCR data Exp. ID 43461

Forward primer (5'-3')

AAGGATAACGAACCAGCAAAGC

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

TTTGAGGAATCCTCAATCCTCG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38679)
Chromosome 7
Position 80.00 cM
Confidence I(LOD2)
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39856

Forward primer (5'-3')

AAGGATAACGAACCAGCAAAGC

Accessions and product sizes

Solanum lycopersicum TA209

1350

Solanum pennellii TA56

1350

Approximate temperature

55°C

Reverse primer (5'-3')

TTTGAGGAATCCTCAATCCTCG

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

500+850

Solanum pennellii TA56

1200

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 26815)
Chromosome 4
Position 13.48 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11498
Forward primer (5'-3') AAGGATAACGAACCAGCAAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTGAGGAATCCTCAATCCTCG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 400	Approximate temperature 55°C

PCR data Exp. ID 11499
Forward primer (5'-3') AAGGATAACGAACCAGCAAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTGAGGAATCCTCAATCCTCG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 400	Approximate temperature 55°C

PCR data Exp. ID 11500
Forward primer (5'-3') AAGGATAACGAACCAGCAAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTGAGGAATCCTCAATCCTCG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 400	Approximate temperature 55°C

PCR data Exp. ID 13472
Forward primer (5'-3') AAGGATAACGAACCAGCAAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTGAGGAATCCTCAATCCTCG	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 500	Approximate temperature 55°C

PCR data Exp. ID 13473
Forward primer (5'-3') AAGGATAACGAACCAGCAAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTGAGGAATCCTCAATCCTCG	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 400	Approximate temperature 55°C

PCR data Exp. ID 13474
Forward primer (5'-3') AAGGATAACGAACCAGCAAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTGAGGAATCCTCAATCCTCG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1100	Approximate temperature 55°C

PCR data Exp. ID 13475
Forward primer (5'-3') AAGGATAACGAACCAGCAAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTGAGGAATCCTCAATCCTCG	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 450	Approximate temperature 55°C

PCR data Exp. ID 13476
Forward primer (5'-3') AAGGATAACGAACCAGCAAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTGAGGAATCCTCAATCCTCG	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 450	Approximate temperature 55°C

PCR data Exp. ID 33114
Forward primer (5'-3') AAGGATAACGAACCAGCAAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTGAGGAATCCTCAATCCTCG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1100	Approximate temperature 55°C

PCR data Exp. ID 11501
Forward primer (5'-3') AAGGATAACGAACCAGCAAAGC	Digested band sizes (using RsaI) Solanum lycopersicum LA925: 400 Solanum pennellii LA716: 300	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTGAGGAATCCTCAATCCTCG	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI,MspI,RsaI,DraI,TaqI,RV,HaeIII,HinfI,KpnI
Accessions and product sizes Solanum pennellii LA716: 400 Solanum lycopersicum LA925: 400	Approximate temperature 55°C