SGN Marker C2_At4g35250

SGN-M8476
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

SynonymsNone
Locus associationsNone
Orthologs in this COSII group 
SpeciesCopiesSequence IDCDS/Edited sequencePeptide sequencePredicted introns
Lycopersicon CombinedSingleSGN-U219338---
Solanum tuberosumSingleSGN-U245624---
ArabidopsisSingleAt4g35250 on TAIR---
Mapped locations  
Map: Tomato-EXPEN 2000   
Map version
52 (location: 38715)
Chromosome
1
Position
165.00 cM
Confidence
I(LOD2)
Protocol
CAPS


Tomato-EXPEN 2000

PCR data   Exp. ID 39885
Forward primer (5'-3')
TCCACCCTCTACGGCCACCTTG
Accessions and product sizes
Solanum pennellii TA56
600
Solanum lycopersicum TA209
600
Approximate temperature
55°C
Reverse primer (5'-3')
TATAGGCTCCTCAGGGCGTCC
Digested band sizes (using unknown enzyme)
Mg+2 concentration
1.5mM
   Enzymes
  

Map: Arabidopsis COSII   
Map version
44 (location: 26921)
Chromosome
4
Position
16.77 MB
Confidence
uncalculated
Protocol
CAPS


Arabidopsis COSII
Other PCR data 
PCR data   Exp. ID 11579
Forward primer (5'-3')
TCCACCCTCTACGGCCACCTTG
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
TATAGGCTCCTCAGGGCGTCC
Enzymes
  
Accessions and product sizes
Solanum pennellii LA716: 700
Approximate temperature
55°C
  
PCR data   Exp. ID 11580
Forward primer (5'-3')
TCCACCCTCTACGGCCACCTTG
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
TATAGGCTCCTCAGGGCGTCC
Enzymes
  
Accessions and product sizes
Solanum lycopersicum LA925: 700
Approximate temperature
55°C
  
PCR data   Exp. ID 11581
Forward primer (5'-3')
TCCACCCTCTACGGCCACCTTG
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
TATAGGCTCCTCAGGGCGTCC
Enzymes
  
Accessions and product sizes
Solanum pimpinellifolium LA1589: 700
Approximate temperature
55°C
  
PCR data   Exp. ID 11582
Forward primer (5'-3')
TCCACCCTCTACGGCCACCTTG
Digested band sizes (using ApoI)
Solanum lycopersicum LA925: 550
Solanum pennellii LA716: 700
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
TATAGGCTCCTCAGGGCGTCC
Enzymes
All possible enzymes for pennellii and and lycopersicum: ApoI,PstI,RsaI
All possible enzymes for lycopersicum and pimpinellifolium: RsaI
  
Accessions and product sizes
Solanum lycopersicum LA925: 700
Solanum pennellii LA716: 700
Approximate temperature
55°C
  
Unigene blast matches for primers 
Universal primers for Asterid species 
No additional PCR data found.
Other COSII sequence data 

Alignment of Arabidopsis CDS and edited Asterid unigenes, FASTA
Alignment of DNA and translated peptides from Arabidopsis CDS and edited Asterid unigenes, plain text
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, ClustalW
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, FASTA

Amplicon sequence alignment, FASTA
At4g35250F.cds.txt, plain text
Forward amplicon sequence for S. pimpinellifolium LA1589, AB1 chromatogram - [View]
Forward amplicon sequence for S. pimpinellifolium LA1589, plain text
Forward amplicon sequence for S. lycopersicum TA209, AB1 chromatogram - [View]
Forward amplicon sequence for S. lycopersicum TA209, plain text
Forward amplicon sequence for S. pennellii TA56, AB1 chromatogram - [View]
Forward amplicon sequence for S. pennellii TA56, plain text
Forward amplicon sequence for S. betaceum 6002061 plant #4, AB1 chromatogram - [View]
Forward amplicon sequence for S. betaceum 6002061 plant #4, plain text
Forward amplicon sequence for S. uniloba 6002078 plant #6, AB1 chromatogram - [View]
Forward amplicon sequence for S. uniloba 6002078 plant #6, plain text

Genomic location of C2_At4g35250  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D8476 marker 8476
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