Sol Genomics Network

SGN-M8476
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Lycopersicon Combined	Single	SGN-U219338	-	-	-
Solanum tuberosum	Single	SGN-U245624	-	-	-
Arabidopsis	Single	At4g35250 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39885

Forward primer (5'-3')

TCCACCCTCTACGGCCACCTTG

Accessions and product sizes

Solanum lycopersicum TA209

600

Solanum pennellii TA56

600

Approximate temperature

55°C

Reverse primer (5'-3')

TATAGGCTCCTCAGGGCGTCC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11579
Forward primer (5'-3') TCCACCCTCTACGGCCACCTTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TATAGGCTCCTCAGGGCGTCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 700	Approximate temperature 55°C

PCR data Exp. ID 11580
Forward primer (5'-3') TCCACCCTCTACGGCCACCTTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TATAGGCTCCTCAGGGCGTCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 700	Approximate temperature 55°C

PCR data Exp. ID 11581
Forward primer (5'-3') TCCACCCTCTACGGCCACCTTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TATAGGCTCCTCAGGGCGTCC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 700	Approximate temperature 55°C

PCR data Exp. ID 11582
Forward primer (5'-3') TCCACCCTCTACGGCCACCTTG	Digested band sizes (using ApoI) Solanum pennellii LA716: 700 Solanum lycopersicum LA925: 550	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TATAGGCTCCTCAGGGCGTCC	Enzymes All possible enzymes for pennellii and and lycopersicum: ApoI,PstI,RsaI All possible enzymes for lycopersicum and pimpinellifolium: RsaI
Accessions and product sizes Solanum lycopersicum LA925: 700 Solanum pennellii LA716: 700	Approximate temperature 55°C