Sol Genomics Network

SGN-M8535
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	300443 (SGN) 123822 (CGN)	-	-	-
Lycopersicon Combined	Single	SGN-U228925	-	-	-
Solanum tuberosum	Single	SGN-U250220	-	-	-
Arabidopsis	Single	At5g01260 on TAIR	-	-	-

Arabidopsis COSII

PCR data Exp. ID 41652

Forward primer (5'-3')

AATGGTCGGAGGGACATGTATGG

Accessions and product sizes

Solanum pennellii TA56

850

Solanum lycopersicum TA209

850

Approximate temperature

55°C

Reverse primer (5'-3')

TGCAGCTCAGCATTGTCCCAATC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

400

Solanum pennellii TA56

850

Mg⁺² concentration

1.5mM

Enzymes

Other PCR data

PCR data Exp. ID 11612
Forward primer (5'-3') AATGGTCGGAGGGACATGTATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCAGCTCAGCATTGTCCCAATC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 250	Approximate temperature 53°C

PCR data Exp. ID 11613
Forward primer (5'-3') AATGGTCGGAGGGACATGTATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCAGCTCAGCATTGTCCCAATC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 250	Approximate temperature 53°C

PCR data Exp. ID 11614
Forward primer (5'-3') AATGGTCGGAGGGACATGTATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCAGCTCAGCATTGTCCCAATC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 250	Approximate temperature 53°C