Sol Genomics Network

SGN-M8672
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	312156 (SGN) 129066 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U200740	-	-	-
Lycopersicon Combined	Multiple	SGN-U225360	-	-	-
Solanum tuberosum	Single	SGN-U253572	-	-	-
Arabidopsis	Single	At5g13030 on TAIR	-	-	-

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46752)
Chromosome 11
Position 90.60 cM
Confidence I(LOD2)
Protocol CAPS

Tobacco N. tomentosiformis

Tomato-EXPEN 2000

PCR data Exp. ID 39924

Forward primer (5'-3')

TCCAAGAACAGATACCATTCCAAGAG

Accessions and product sizes

Solanum lycopersicum TA209

150

Solanum pennellii TA56

150

Approximate temperature

55°C

Reverse primer (5'-3')

AACAGAAGGCAACATTTTCGTATAGC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11707
Forward primer (5'-3') TCCAAGAACAGATACCATTCCAAGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACAGAAGGCAACATTTTCGTATAGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1650	Approximate temperature 55°C

PCR data Exp. ID 11708
Forward primer (5'-3') TCCAAGAACAGATACCATTCCAAGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACAGAAGGCAACATTTTCGTATAGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1650	Approximate temperature 55°C

PCR data Exp. ID 11709
Forward primer (5'-3') TCCAAGAACAGATACCATTCCAAGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACAGAAGGCAACATTTTCGTATAGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1650	Approximate temperature 55°C

PCR data Exp. ID 13708
Forward primer (5'-3') TCCAAGAACAGATACCATTCCAAGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACAGAAGGCAACATTTTCGTATAGC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 2000	Approximate temperature 55°C

PCR data Exp. ID 13709
Forward primer (5'-3') TCCAAGAACAGATACCATTCCAAGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACAGAAGGCAACATTTTCGTATAGC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1200	Approximate temperature 55°C

PCR data Exp. ID 13710
Forward primer (5'-3') TCCAAGAACAGATACCATTCCAAGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACAGAAGGCAACATTTTCGTATAGC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1200	Approximate temperature 55°C

PCR data Exp. ID 13711
Forward primer (5'-3') TCCAAGAACAGATACCATTCCAAGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACAGAAGGCAACATTTTCGTATAGC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1200	Approximate temperature 55°C

PCR data Exp. ID 32654
Forward primer (5'-3') TCCAAGAACAGATACCATTCCAAGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACAGAAGGCAACATTTTCGTATAGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1500	Approximate temperature 55°C

PCR data Exp. ID 33202
Forward primer (5'-3') TCCAAGAACAGATACCATTCCAAGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACAGAAGGCAACATTTTCGTATAGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1500	Approximate temperature 55°C

PCR data Exp. ID 33203
Forward primer (5'-3') TCCAAGAACAGATACCATTCCAAGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACAGAAGGCAACATTTTCGTATAGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1500	Approximate temperature 55°C

PCR data Exp. ID 33204
Forward primer (5'-3') TCCAAGAACAGATACCATTCCAAGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACAGAAGGCAACATTTTCGTATAGC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1200	Approximate temperature 55°C

PCR data Exp. ID 33205
Forward primer (5'-3') TCCAAGAACAGATACCATTCCAAGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACAGAAGGCAACATTTTCGTATAGC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1200	Approximate temperature 55°C

PCR data Exp. ID 33206
Forward primer (5'-3') TCCAAGAACAGATACCATTCCAAGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACAGAAGGCAACATTTTCGTATAGC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1200	Approximate temperature 55°C

PCR data Exp. ID 11710
Forward primer (5'-3') TCCAAGAACAGATACCATTCCAAGAG	Digested band sizes (using MspI) Solanum lycopersicum LA925: 750+900 Solanum pennellii LA716: 750+850	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AACAGAAGGCAACATTTTCGTATAGC	Enzymes All possible enzymes for pennellii and and lycopersicum: MspI All possible enzymes for lycopersicum and pimpinellifolium: MspI
Accessions and product sizes Solanum pennellii LA716: 1650 Solanum lycopersicum LA925: 1650	Approximate temperature 55°C