Sol Genomics Network

SGN-M8686
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	302332 (SGN) 121512 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U202265	-	-	-
Solanum tuberosum	Single	SGN-U255612	-	-	-
Arabidopsis	Single	At5g13640 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39929

Forward primer (5'-3')

TCAGCTGAAGCACGGGATATTGC

Accessions and product sizes

Solanum pennellii TA56

920+1350

Solanum lycopersicum TA209

920+1350

Approximate temperature

55°C

Reverse primer (5'-3')

TGGAACATATCCGTGTCCAGAACACC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

1350+640

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11721
Forward primer (5'-3') TCAGCTGAAGCACGGGATATTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGAACATATCCGTGTCCAGAACACC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 650	Approximate temperature 55°C

PCR data Exp. ID 11722
Forward primer (5'-3') TCAGCTGAAGCACGGGATATTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGAACATATCCGTGTCCAGAACACC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 650	Approximate temperature 55°C

PCR data Exp. ID 11723
Forward primer (5'-3') TCAGCTGAAGCACGGGATATTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGAACATATCCGTGTCCAGAACACC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 650	Approximate temperature 55°C

PCR data Exp. ID 13724
Forward primer (5'-3') TCAGCTGAAGCACGGGATATTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGAACATATCCGTGTCCAGAACACC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 650	Approximate temperature 55°C

PCR data Exp. ID 13725
Forward primer (5'-3') TCAGCTGAAGCACGGGATATTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGAACATATCCGTGTCCAGAACACC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 650	Approximate temperature 55°C

PCR data Exp. ID 13726
Forward primer (5'-3') TCAGCTGAAGCACGGGATATTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGAACATATCCGTGTCCAGAACACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 650	Approximate temperature 55°C

PCR data Exp. ID 13727
Forward primer (5'-3') TCAGCTGAAGCACGGGATATTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGAACATATCCGTGTCCAGAACACC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 650	Approximate temperature 55°C

PCR data Exp. ID 13728
Forward primer (5'-3') TCAGCTGAAGCACGGGATATTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGAACATATCCGTGTCCAGAACACC	Enzymes
Accessions and product sizes Physalis TA1367: 650	Approximate temperature 55°C

PCR data Exp. ID 13729
Forward primer (5'-3') TCAGCTGAAGCACGGGATATTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGAACATATCCGTGTCCAGAACACC	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 650	Approximate temperature 55°C

PCR data Exp. ID 13730
Forward primer (5'-3') TCAGCTGAAGCACGGGATATTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGAACATATCCGTGTCCAGAACACC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 650	Approximate temperature 55°C

PCR data Exp. ID 33211
Forward primer (5'-3') TCAGCTGAAGCACGGGATATTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGAACATATCCGTGTCCAGAACACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 650	Approximate temperature 55°C

PCR data Exp. ID 33212
Forward primer (5'-3') TCAGCTGAAGCACGGGATATTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGAACATATCCGTGTCCAGAACACC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 650	Approximate temperature 55°C

PCR data Exp. ID 11724
Forward primer (5'-3') TCAGCTGAAGCACGGGATATTGC	Digested band sizes (using TaqI) Solanum pennellii LA716: 420 Solanum lycopersicum LA925: 550	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGAACATATCCGTGTCCAGAACACC	Enzymes All possible enzymes for pennellii and and lycopersicum: TaqI
Accessions and product sizes Solanum pennellii LA716: 650 Solanum lycopersicum LA925: 650	Approximate temperature 55°C