Sol Genomics Network

SGN Marker C2_At5g20180

SGN-M8779
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	301390 (SGN) 125300 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U198242	-	-	-
Lycopersicon Combined	Single	SGN-U220271	-	-	-
Solanum tuberosum	Single	SGN-U250312	-	-	-
Arabidopsis	Single	At5g20180 on TAIR	-	-	-

Mapped locations

Map: Tomato-EXPEN 2000
Map version 52 (location: 38807)
Chromosome 7
Position 6.00 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39940

Forward primer (5'-3')

TGCTATGTACATCTAATCCCAAGCAC

Accessions and product sizes

Solanum pennellii TA56

1300

Solanum lycopersicum TA209

1300

Approximate temperature

55°C

Reverse primer (5'-3')

AGCTATCCCCCTTTTCCACCAAG

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

1300

Solanum pennellii TA56

430+520

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 27239)
Chromosome 5
Position 6.81 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11767
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1300	Approximate temperature 55°C

PCR data Exp. ID 11768
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1300	Approximate temperature 55°C

PCR data Exp. ID 11769
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1300	Approximate temperature 55°C

PCR data Exp. ID 13749
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 1300	Approximate temperature 55°C

PCR data Exp. ID 13750
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Solanum melongena TA2986: 1050	Approximate temperature 55°C

PCR data Exp. ID 13751
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 1300	Approximate temperature 55°C

PCR data Exp. ID 13752
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1200	Approximate temperature 55°C

PCR data Exp. ID 13753
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 1200	Approximate temperature 55°C

PCR data Exp. ID 13754
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1050	Approximate temperature 55°C

PCR data Exp. ID 13755
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1050	Approximate temperature 55°C

PCR data Exp. ID 13756
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Physalis TA1367: Multiple	Approximate temperature 55°C

PCR data Exp. ID 13757
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 1300	Approximate temperature 55°C

PCR data Exp. ID 13758
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: Multiple	Approximate temperature 55°C

PCR data Exp. ID 13759
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 1350	Approximate temperature 55°C

PCR data Exp. ID 13760
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 1300	Approximate temperature 55°C

PCR data Exp. ID 33220
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1200	Approximate temperature 55°C

PCR data Exp. ID 33221
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 1200	Approximate temperature 55°C

PCR data Exp. ID 33222
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1050	Approximate temperature 55°C

PCR data Exp. ID 33223
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1050	Approximate temperature 55°C

PCR data Exp. ID 11770
Forward primer (5'-3') TGCTATGTACATCTAATCCCAAGCAC	Digested band sizes (using HinfI) Solanum pennellii LA716: 430+520 Solanum lycopersicum LA925: 1300	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTATCCCCCTTTTCCACCAAG	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI, DraI, HinfI All possible enzymes for lycopersicum and pimpinellifolium: AluI, TaqI
Accessions and product sizes Solanum lycopersicum LA925: 1300 Solanum pennellii LA716: 1300	Approximate temperature 55°C