SGN Marker C2_At5g20350

SGN-M8781
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

SynonymsNone
Locus associationsNone
Orthologs in this COSII group 
SpeciesCopiesSequence IDCDS/Edited sequencePeptide sequencePredicted introns
CoffeeSingle304405 (SGN)
124130 (CGN)
---
Capsicum annuumSingleSGN-U202994---
Lycopersicon CombinedMultipleSGN-U222569---
Solanum tuberosumSingleSGN-U249534---
ArabidopsisSingleAt5g20350 on TAIR---
Mapped locations  
Map: Tomato-EXPEN 2000   
Map version
52 (location: 38808)
Chromosome
7
Position
0.00 cM
Confidence
I
Protocol
CAPS


Tomato-EXPEN 2000

PCR data   Exp. ID 39941
Forward primer (5'-3')
TCGGAGATCGAGGTGGTGGAGG
Accessions and product sizes
Solanum pennellii TA56
750+1000
Solanum lycopersicum TA209
750
Approximate temperature
55°C
Reverse primer (5'-3')
AGAGCTCAGCAACCTGCACTGCAC
Digested band sizes (using unknown enzyme)
Mg+2 concentration
1.5mM
   Enzymes
  

Map: Arabidopsis COSII   
Map version
44 (location: 27241)
Chromosome
5
Position
6.88 MB
Confidence
uncalculated
Protocol
CAPS


Arabidopsis COSII
Other PCR data 
PCR data   Exp. ID 11771
Forward primer (5'-3')
TCGGAGATCGAGGTGGTGGAGG
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
AGAGCTCAGCAACCTGCACTGCAC
Enzymes
  
Accessions and product sizes
Solanum pennellii LA716: 1000
Approximate temperature
55°C
  
PCR data   Exp. ID 11772
Forward primer (5'-3')
TCGGAGATCGAGGTGGTGGAGG
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
AGAGCTCAGCAACCTGCACTGCAC
Enzymes
  
Accessions and product sizes
Solanum lycopersicum LA925: 1000
Approximate temperature
55°C
  
PCR data   Exp. ID 11773
Forward primer (5'-3')
TCGGAGATCGAGGTGGTGGAGG
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
AGAGCTCAGCAACCTGCACTGCAC
Enzymes
  
Accessions and product sizes
Solanum pimpinellifolium LA1589: 1000
Approximate temperature
55°C
  
PCR data   Exp. ID 11774
Forward primer (5'-3')
TCGGAGATCGAGGTGGTGGAGG
Digested band sizes (using BslI)
Solanum pennellii LA716: 800
Solanum lycopersicum LA925: 650
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
AGAGCTCAGCAACCTGCACTGCAC
Enzymes
All possible enzymes for pennellii and and lycopersicum: ApoI,BslI,BstUI,BstXI,HinfI,PflMI,StyI
All possible enzymes for lycopersicum and pimpinellifolium: ApoI,BslI,BstXI,EcoRI,PflMI
  
Accessions and product sizes
Solanum lycopersicum LA925: 1000
Solanum pennellii LA716: 1000
Approximate temperature
55°C
  
Unigene blast matches for primers 
Universal primers for Asterid species 
No additional PCR data found.
Other COSII sequence data 

Alignment of Arabidopsis CDS and edited Asterid unigenes, FASTA
Alignment of DNA and translated peptides from Arabidopsis CDS and edited Asterid unigenes, plain text
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, ClustalW
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, FASTA

Amplicon sequence alignment, FASTA
At5g20350F.cds.txt, plain text
Forward amplicon sequence for S. pimpinellifolium LA1589, AB1 chromatogram - [View]
Forward amplicon sequence for S. pimpinellifolium LA1589, PDF chromatogram
Forward amplicon sequence for S. pimpinellifolium LA1589, plain text
Forward amplicon sequence for S. lycopersicum TA209, AB1 chromatogram - [View]
Forward amplicon sequence for S. lycopersicum TA209, PDF chromatogram
Forward amplicon sequence for S. lycopersicum TA209, plain text
Forward amplicon sequence for S. pennellii TA56, AB1 chromatogram - [View]
Forward amplicon sequence for S. pennellii TA56, PDF chromatogram
Forward amplicon sequence for S. pennellii TA56, plain text

Genomic location of C2_At5g20350  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D8781 marker 8781
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