Sol Genomics Network

SGN-M8781
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	304405 (SGN) 124130 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U202994	-	-	-
Lycopersicon Combined	Multiple	SGN-U222569	-	-	-
Solanum tuberosum	Single	SGN-U249534	-	-	-
Arabidopsis	Single	At5g20350 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39941

Forward primer (5'-3')

TCGGAGATCGAGGTGGTGGAGG

Accessions and product sizes

Solanum lycopersicum TA209

750

Solanum pennellii TA56

1000+750

Approximate temperature

55°C

Reverse primer (5'-3')

AGAGCTCAGCAACCTGCACTGCAC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11771
Forward primer (5'-3') TCGGAGATCGAGGTGGTGGAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGAGCTCAGCAACCTGCACTGCAC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1000	Approximate temperature 55°C

PCR data Exp. ID 11772
Forward primer (5'-3') TCGGAGATCGAGGTGGTGGAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGAGCTCAGCAACCTGCACTGCAC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1000	Approximate temperature 55°C

PCR data Exp. ID 11773
Forward primer (5'-3') TCGGAGATCGAGGTGGTGGAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGAGCTCAGCAACCTGCACTGCAC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1000	Approximate temperature 55°C

PCR data Exp. ID 11774
Forward primer (5'-3') TCGGAGATCGAGGTGGTGGAGG	Digested band sizes (using BslI) Solanum pennellii LA716: 800 Solanum lycopersicum LA925: 650	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGAGCTCAGCAACCTGCACTGCAC	Enzymes All possible enzymes for pennellii and and lycopersicum: ApoI,BslI,BstUI,BstXI,HinfI,PflMI,StyI All possible enzymes for lycopersicum and pimpinellifolium: ApoI,BslI,BstXI,EcoRI,PflMI
Accessions and product sizes Solanum pennellii LA716: 1000 Solanum lycopersicum LA925: 1000	Approximate temperature 55°C