Sol Genomics Network

SGN Marker C2_At5g23880

SGN-M8824
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	303097 (SGN) 122556 (CGN)	-	-	-
Lycopersicon Combined	Single	SGN-U235932	-	-	-
Arabidopsis	Single	At5g23880 on TAIR	-	-	-

Mapped locations

Map: Eggplant-COSII
Map version 63 (location: 42253)
Chromosome 3
Position 65.70 cM
Confidence F(LOD3)
Protocol dCAPS

Eggplant-COSII

PCR data Exp. ID 43821

Forward primer (5'-3')

AGCTAACCTAATCCTTGATACAACACC

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

TCTATTCTCATTTGTTCATGTTGAAGGTA

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Pepper-COSII
Map version 58 (location: 41525)
Chromosome 3
Position 71.10 cM
Confidence I(LOD2)
Protocol CAPS

Pepper-COSII

PCR data Exp. ID 43501

Forward primer (5'-3')

AGCTAACCTAATCCTTGATACAACACC

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

ACCATCAGAACGACCTTCGAAGTCC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38821)
Chromosome 3
Position 53.50 cM
Confidence I(LOD2)
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39947

Forward primer (5'-3')

AGCTAACCTAATCCTTGATACAACACC

Accessions and product sizes

Solanum lycopersicum TA209

400

Solanum pennellii TA56

400

Approximate temperature

55°C

Reverse primer (5'-3')

ACCATCAGAACGACCTTCGAAGTCC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 27285)
Chromosome 5
Position 8.06 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11811
Forward primer (5'-3') AGCTAACCTAATCCTTGATACAACACC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCATCAGAACGACCTTCGAAGTCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1650	Approximate temperature 55°C

PCR data Exp. ID 32578
Forward primer (5'-3') AGCTAACCTAATCCTTGATACAACACC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCATCAGAACGACCTTCGAAGTCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1650	Approximate temperature 53°C

PCR data Exp. ID 32579
Forward primer (5'-3') AGCTAACCTAATCCTTGATACAACACC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCATCAGAACGACCTTCGAAGTCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1650	Approximate temperature 53°C

PCR data Exp. ID 32580
Forward primer (5'-3') AGCTAACCTAATCCTTGATACAACACC	Digested band sizes (using TaqI) Solanum pennellii LA716: 400+1150 Solanum lycopersicum LA925: 500+1150	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCATCAGAACGACCTTCGAAGTCC	Enzymes All possible enzymes for pennellii and and lycopersicum: BanII,BsiEI,TaqI
Accessions and product sizes Solanum lycopersicum LA925: 1650 Solanum pennellii LA716: 1650	Approximate temperature 53°C