Sol Genomics Network

SGN Marker C2_At5g25630

SGN-M8842
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	303207 (SGN) 120288 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U201026	-	-	-
Lycopersicon Combined	Single	SGN-U223916	-	-	-
Solanum tuberosum	Single	SGN-U251940	-	-	-
Arabidopsis	Single	At5g25630 on TAIR	-	-	-

Mapped locations

Map: Solanum michoacanum 2011
Map version 155 (location: 109245)
Chromosome 8
Position 29.72 cM
Confidence uncalculated
Protocol CAPS

Solanum michoacanum 2011

Map: Tobacco N. acuminata
Map version 89 (location: 46531)
Chromosome 8
Position 70.00 cM
Confidence I(LOD2)
Protocol CAPS

Tobacco N. acuminata

Map: Pepper-COSII
Map version 58 (location: 41526)
Chromosome 1
Position 170.00 cM
Confidence I
Protocol dCAPS

Pepper-COSII

PCR data Exp. ID 43502

Forward primer (5'-3')

TTTCAATTCACTGATCAAAGGTTTCC

Accessions and product sizes

Approximate temperature

44°C

Reverse primer (5'-3')

CCATCCTAAGACCTAAAGAAGTATATAGA

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38827)
Chromosome 8
Position 41.50 cM
Confidence F(LOD3)
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39948

Forward primer (5'-3')

TTTCAATTCACTGATCAAAGGTTTCC

Accessions and product sizes

Solanum lycopersicum TA209

300

Solanum pennellii TA56

550

Approximate temperature

55°C

Reverse primer (5'-3')

ACCTCCCTGCACTTAACCATATATCC

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

550

Solanum lycopersicum TA209

300

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 27303)
Chromosome 5
Position 8.95 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11815
Forward primer (5'-3') TTTCAATTCACTGATCAAAGGTTTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCTCCCTGCACTTAACCATATATCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1000	Approximate temperature 53°C

PCR data Exp. ID 11816
Forward primer (5'-3') TTTCAATTCACTGATCAAAGGTTTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCTCCCTGCACTTAACCATATATCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1650	Approximate temperature 53°C

PCR data Exp. ID 11817
Forward primer (5'-3') TTTCAATTCACTGATCAAAGGTTTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCTCCCTGCACTTAACCATATATCC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1650	Approximate temperature 53°C

PCR data Exp. ID 13808
Forward primer (5'-3') TTTCAATTCACTGATCAAAGGTTTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCTCCCTGCACTTAACCATATATCC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: Multiple	Approximate temperature 55°C

PCR data Exp. ID 13809
Forward primer (5'-3') TTTCAATTCACTGATCAAAGGTTTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCTCCCTGCACTTAACCATATATCC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 900	Approximate temperature 55°C

PCR data Exp. ID 13810
Forward primer (5'-3') TTTCAATTCACTGATCAAAGGTTTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCTCCCTGCACTTAACCATATATCC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1900	Approximate temperature 55°C

PCR data Exp. ID 13811
Forward primer (5'-3') TTTCAATTCACTGATCAAAGGTTTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCTCCCTGCACTTAACCATATATCC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 1900	Approximate temperature 55°C

PCR data Exp. ID 32657
Forward primer (5'-3') TTTCAATTCACTGATCAAAGGTTTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCTCCCTGCACTTAACCATATATCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 900	Approximate temperature 55°C

PCR data Exp. ID 33241
Forward primer (5'-3') TTTCAATTCACTGATCAAAGGTTTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCTCCCTGCACTTAACCATATATCC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 600	Approximate temperature 55°C

PCR data Exp. ID 33242
Forward primer (5'-3') TTTCAATTCACTGATCAAAGGTTTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCTCCCTGCACTTAACCATATATCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 900	Approximate temperature 55°C

PCR data Exp. ID 33243
Forward primer (5'-3') TTTCAATTCACTGATCAAAGGTTTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCTCCCTGCACTTAACCATATATCC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 900	Approximate temperature 55°C

PCR data Exp. ID 33244
Forward primer (5'-3') TTTCAATTCACTGATCAAAGGTTTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCTCCCTGCACTTAACCATATATCC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1900	Approximate temperature 55°C

PCR data Exp. ID 11818
Forward primer (5'-3') TTTCAATTCACTGATCAAAGGTTTCC	Digested band sizes (using amplicon difference) Solanum lycopersicum LA925: 1650 Solanum pennellii LA716: 1000	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCTCCCTGCACTTAACCATATATCC	Enzymes All possible enzymes for pennellii and and lycopersicum: amplicon difference All possible enzymes for lycopersicum and pimpinellifolium: HinfI
Accessions and product sizes Solanum lycopersicum LA925: 1650 Solanum pennellii LA716: 1000	Approximate temperature 53°C