Sol Genomics Network

SGN-M8857
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Lycopersicon Combined	Single	SGN-U217731	-	-	-
Solanum tuberosum	Single	SGN-U251807	-	-	-
Arabidopsis	Single	At5g27390 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39954

Forward primer (5'-3')

ATGGCCATGTCCACGCTCCTC

Accessions and product sizes

Solanum lycopersicum TA209

230

Solanum pennellii TA56

230

Approximate temperature

53°C

Reverse primer (5'-3')

TGGGCTTAGCCTTATCTCCATATAG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11837
Forward primer (5'-3') ATGGCCATGTCCACGCTCCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGGCTTAGCCTTATCTCCATATAG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 900	Approximate temperature 55°C

PCR data Exp. ID 11838
Forward primer (5'-3') ATGGCCATGTCCACGCTCCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGGCTTAGCCTTATCTCCATATAG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 900	Approximate temperature 55°C

PCR data Exp. ID 11839
Forward primer (5'-3') ATGGCCATGTCCACGCTCCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGGCTTAGCCTTATCTCCATATAG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 900	Approximate temperature 55°C

PCR data Exp. ID 11840
Forward primer (5'-3') ATGGCCATGTCCACGCTCCTC	Digested band sizes (using DpnII) Solanum pennellii LA716: 300+600 Solanum lycopersicum LA925: 400+500	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGGCTTAGCCTTATCTCCATATAG	Enzymes All possible enzymes for pennellii and and lycopersicum: BstYI,DpnII,HincII,HinfI,RsaI,TspRI All possible enzymes for lycopersicum and pimpinellifolium: DpnII,RsaI
Accessions and product sizes Solanum pennellii LA716: 900 Solanum lycopersicum LA925: 900	Approximate temperature 55°C