Sol Genomics Network

SGN Marker C2_At5g38530

SGN-M8896
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Lycopersicon Combined	Single	SGN-U215849	-	-	-
Solanum tuberosum	Single	SGN-U249761	-	-	-
Arabidopsis	Single	At5g38530 on TAIR	-	-	-

Mapped locations

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46767)
Chromosome 12
Position 33.40 cM
Confidence F(LOD3)
Protocol CAPS

Tobacco N. tomentosiformis

Map: Pepper-COSII
Map version 58 (location: 41530)
Chromosome 12
Position 98.00 cM
Confidence F(LOD3)
Protocol dCAPS

Pepper-COSII

PCR data Exp. ID 43506

Forward primer (5'-3')

TTGTATTGTGAATCGCCTATATGT

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

ACTCCTCACCGATAACGGTCTGATG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38850)
Chromosome 12
Position 93.00 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39960

Forward primer (5'-3')

TCGCACAAGCCCAACTCTGCTG

Accessions and product sizes

Solanum pennellii TA56

700

Solanum lycopersicum TA209

700

Approximate temperature

55°C

Reverse primer (5'-3')

ACTCCTCACCGATAACGGTCTGATG

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

700

Solanum lycopersicum TA209

550

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 27361)
Chromosome 5
Position 15.44 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11869
Forward primer (5'-3') TCGCACAAGCCCAACTCTGCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTCCTCACCGATAACGGTCTGATG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 450	Approximate temperature 55°C

PCR data Exp. ID 11870
Forward primer (5'-3') TCGCACAAGCCCAACTCTGCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTCCTCACCGATAACGGTCTGATG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 450	Approximate temperature 55°C

PCR data Exp. ID 11871
Forward primer (5'-3') TCGCACAAGCCCAACTCTGCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTCCTCACCGATAACGGTCTGATG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 450	Approximate temperature 55°C

PCR data Exp. ID 11872
Forward primer (5'-3') TCGCACAAGCCCAACTCTGCTG	Digested band sizes (using TspRI) Solanum pennellii LA716: 410 Solanum lycopersicum LA925: 450	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTCCTCACCGATAACGGTCTGATG	Enzymes All possible enzymes for pennellii and and lycopersicum: AvaII,BclI,BglI,DpnII,HinfI,RsaI,Sau96I,TspRI,XcmI All possible enzymes for lycopersicum and pimpinellifolium: MspI,NciI,ScrFI
Accessions and product sizes Solanum lycopersicum LA925: 450 Solanum pennellii LA716: 450	Approximate temperature 55°C