Sol Genomics Network

SGN-M9019
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	307713 (SGN) 129770 (CGN)	-	-	-
Lycopersicon Combined	Single	SGN-U224664	-	-	-
Solanum tuberosum	Single	SGN-U257675	-	-	-
Arabidopsis	Single	At5g49880 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39983

Forward primer (5'-3')

TCGGCTCCGTTGCCGGAATC

Accessions and product sizes

Solanum lycopersicum TA209

1000

Approximate temperature

53°C

Reverse primer (5'-3')

ACTGCTTAAGGCATCAAAAAACTC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11970
Forward primer (5'-3') TCGGCTCCGTTGCCGGAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTGCTTAAGGCATCAAAAAACTC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 850	Approximate temperature 55°C

PCR data Exp. ID 11971
Forward primer (5'-3') TCGGCTCCGTTGCCGGAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTGCTTAAGGCATCAAAAAACTC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 850	Approximate temperature 55°C

PCR data Exp. ID 11972
Forward primer (5'-3') TCGGCTCCGTTGCCGGAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTGCTTAAGGCATCAAAAAACTC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 900	Approximate temperature 55°C

PCR data Exp. ID 32584
Forward primer (5'-3') TCGGCTCCGTTGCCGGAATC	Digested band sizes (using RsaI) Solanum pennellii LA716: 850 Solanum lycopersicum LA925: 650	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTGCTTAAGGCATCAAAAAACTC	Enzymes All possible enzymes for pennellii and and lycopersicum: HincII, RsaI
Accessions and product sizes Solanum lycopersicum LA925: 850 Solanum pennellii LA716: 850	Approximate temperature 55°C