Sol Genomics Network

SGN Marker C2_At5g49970

SGN-M9022
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	308750 (SGN) 127500 (CGN)	-	-	-
Lycopersicon Combined	Multiple	SGN-U218657	-	-	-
Solanum tuberosum	Single	SGN-U255980	-	-	-
Arabidopsis	Single	At5g49970 on TAIR	-	-	-

Mapped locations

Map: Eggplant-COSII
Map version 63 (location: 42260)
Chromosome 3
Position 113.20 cM
Confidence F(LOD3)
Protocol CAPS

Eggplant-COSII

PCR data Exp. ID 43829

Forward primer (5'-3')

AGGTAGATTTTAGTAGGGGAAATCC

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

TCCCACCATTGTTACCAGGACCAC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Pepper-COSII
Map version 58 (location: 41540)
Chromosome 3
Position 114.40 cM
Confidence I
Protocol CAPS

Pepper-COSII

PCR data Exp. ID 43516

Forward primer (5'-3')

AATTGGCAGGCTTGAGTGTTGC

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

TCCCACCATTGTTACCAGGACCAC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38882)
Chromosome 3
Position 99.50 cM
Confidence I(LOD2)
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39984

Forward primer (5'-3')

AATTGGCAGGCTTGAGTGTTGC

Accessions and product sizes

Solanum pennellii TA56

100

Solanum lycopersicum TA209

100

Approximate temperature

55°C

Reverse primer (5'-3')

TCCCACCATTGTTACCAGGACCAC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 27490)
Chromosome 5
Position 20.35 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11975
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 580+750	Approximate temperature 55°C

PCR data Exp. ID 11976
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 580+750	Approximate temperature 55°C

PCR data Exp. ID 11977
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 580+750	Approximate temperature 55°C

PCR data Exp. ID 13880
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 700+400	Approximate temperature 55°C

PCR data Exp. ID 13881
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 680	Approximate temperature 55°C

PCR data Exp. ID 13882
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 750	Approximate temperature 55°C

PCR data Exp. ID 13883
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1200+400	Approximate temperature 55°C

PCR data Exp. ID 13884
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: Multiple	Approximate temperature 55°C

PCR data Exp. ID 13885
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 650+400	Approximate temperature 55°C

PCR data Exp. ID 13886
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 650+400	Approximate temperature 55°C

PCR data Exp. ID 13887
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Physalis TA1367: 920+750	Approximate temperature 55°C

PCR data Exp. ID 13888
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 700+150	Approximate temperature 55°C

PCR data Exp. ID 13889
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 700+150	Approximate temperature 55°C

PCR data Exp. ID 13890
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Coffea canephora BP409: 1300	Approximate temperature 55°C

PCR data Exp. ID 32659
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 480+700	Approximate temperature 55°C

PCR data Exp. ID 33271
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 480+700	Approximate temperature 55°C

PCR data Exp. ID 33272
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 480+700	Approximate temperature 55°C

PCR data Exp. ID 33273
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1200+400	Approximate temperature 55°C

PCR data Exp. ID 33274
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: Multiple	Approximate temperature 55°C

PCR data Exp. ID 33275
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 650+400	Approximate temperature 55°C

PCR data Exp. ID 33276
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 650+400	Approximate temperature 55°C

PCR data Exp. ID 11978
Forward primer (5'-3') AATTGGCAGGCTTGAGTGTTGC	Digested band sizes (using TaqI) Solanum lycopersicum LA925: 410+510 Solanum pennellii LA716: 510+640	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCCACCATTGTTACCAGGACCAC	Enzymes All possible enzymes for pennellii and and lycopersicum: DraI, TaqI, HinfI, DpanII
Accessions and product sizes Solanum pennellii LA716: 580+750 Solanum lycopersicum LA925: 580+750	Approximate temperature 55°C