Sol Genomics Network

SGN Marker C2_At5g50720

SGN-M9027
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	304970 (SGN) 122188 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U200558	-	-	-
Solanum tuberosum	Single	SGN-U248890	-	-	-
Arabidopsis	Single	At5g50720 on TAIR	-	-	-

Mapped locations

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46775)
Chromosome 5
Position 84.90 cM
Confidence F(LOD3)
Protocol PCR

Tobacco N. tomentosiformis

Map: Tomato-EXPEN 2000
Map version 52 (location: 38883)
Chromosome 4
Position 37.50 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39985

Forward primer (5'-3')

TTGGATGATGAACAATGGCTTGC

Accessions and product sizes

Solanum pennellii TA56

450

Solanum lycopersicum TA209

450

Approximate temperature

55°C

Reverse primer (5'-3')

TGAATTGTGGAAAAACTAGCCATGC

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

450

Solanum lycopersicum TA209

410

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 27495)
Chromosome 5
Position 20.65 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11979
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 250	Approximate temperature 55°C

PCR data Exp. ID 11980
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 220	Approximate temperature 55°C

PCR data Exp. ID 11981
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 220	Approximate temperature 55°C

PCR data Exp. ID 13891
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 950	Approximate temperature 55°C

PCR data Exp. ID 13892
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 950+250	Approximate temperature 55°C

PCR data Exp. ID 13893
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 800+580	Approximate temperature 55°C

PCR data Exp. ID 13894
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 800+580	Approximate temperature 55°C

PCR data Exp. ID 13895
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 250	Approximate temperature 55°C

PCR data Exp. ID 13896
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 250	Approximate temperature 55°C

PCR data Exp. ID 13897
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Physalis TA1367: Multiple	Approximate temperature 55°C

PCR data Exp. ID 13898
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 450	Approximate temperature 55°C

PCR data Exp. ID 13899
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 400	Approximate temperature 55°C

PCR data Exp. ID 32660
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 220+750	Approximate temperature 55°C

PCR data Exp. ID 33277
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 220+700	Approximate temperature 55°C

PCR data Exp. ID 33278
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 250+750	Approximate temperature 55°C

PCR data Exp. ID 33279
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 800+580	Approximate temperature 55°C

PCR data Exp. ID 33280
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 800+580	Approximate temperature 55°C

PCR data Exp. ID 33281
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 250	Approximate temperature 55°C

PCR data Exp. ID 33282
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 250	Approximate temperature 55°C

PCR data Exp. ID 11982
Forward primer (5'-3') TTGGATGATGAACAATGGCTTGC	Digested band sizes (using amplicon difference) Solanum lycopersicum LA925: 220 Solanum pennellii LA716: 250	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATTGTGGAAAAACTAGCCATGC	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI,MspI,RsaI,DraI,TaqI,RV,HaeIII,HinfI,DpnII,Kpni All possible enzymes for lycopersicum and pimpinellifolium: amplicon difference
Accessions and product sizes Solanum lycopersicum LA925: 220 Solanum pennellii LA716: 250	Approximate temperature 55°C