Sol Genomics Network

SGN Marker C2_At5g56940

SGN-M9096
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	309065 (SGN) 131510 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U197233	-	-	-
Lycopersicon Combined	Single	SGN-U224510	-	-	-
Arabidopsis	Single	At5g56940 on TAIR	-	-	-

Mapped locations

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46780)
Chromosome 6
Position 48.20 cM
Confidence CF(LOD3)
Protocol PCR

Tobacco N. tomentosiformis

Map: Eggplant-COSII
Map version 63 (location: 42263)
Chromosome 7
Position 54.00 cM
Confidence F(LOD3)
Protocol dCAPS

Eggplant-COSII

PCR data Exp. ID 43832

Forward primer (5'-3')

TTATGCTATTTTTCATTGGAAATTAAT

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

TACTTCAACCTATCAAAATTAAGACC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38894)
Chromosome 7
Position 11.00 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39996

Forward primer (5'-3')

TAGCAGATCTCCGAGAGACGGC

Accessions and product sizes

Solanum lycopersicum TA209

250

Solanum pennellii TA56

250

Approximate temperature

55°C

Reverse primer (5'-3')

TACTTCAACCTATCAAAATTAAGACC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

210

Solanum pennellii TA56

190

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 27569)
Chromosome 5
Position 23.05 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 12031
Forward primer (5'-3') TAGCAGATCTCCGAGAGACGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TACTTCAACCTATCAAAATTAAGACC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 350	Approximate temperature 55°C

PCR data Exp. ID 12032
Forward primer (5'-3') TAGCAGATCTCCGAGAGACGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TACTTCAACCTATCAAAATTAAGACC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 350	Approximate temperature 55°C

PCR data Exp. ID 12033
Forward primer (5'-3') TAGCAGATCTCCGAGAGACGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TACTTCAACCTATCAAAATTAAGACC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 350	Approximate temperature 55°C

PCR data Exp. ID 12034
Forward primer (5'-3') TAGCAGATCTCCGAGAGACGGC	Digested band sizes (using NsiI) Solanum pennellii LA716: 250 Solanum lycopersicum LA925: 350	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TACTTCAACCTATCAAAATTAAGACC	Enzymes All possible enzymes for pennellii and and lycopersicum: NsiI
Accessions and product sizes Solanum pennellii LA716: 350 Solanum lycopersicum LA925: 350	Approximate temperature 55°C