Sol Genomics Network

SGN-M9175
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	304806 (SGN) 124999 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U204250	-	-	-
Solanum tuberosum	Single	SGN-U252775	-	-	-
Arabidopsis	Single	At5g63380 on TAIR	-	-	-

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46787)
Chromosome 3
Position 52.20 cM
Confidence CF(LOD3)
Protocol CAPS

Tobacco N. tomentosiformis

Tomato-EXPEN 2000

PCR data Exp. ID 40016

Forward primer (5'-3')

AGCAGGGGAGATTCCTATGGC

Accessions and product sizes

Solanum pennellii TA56

850

Solanum lycopersicum TA209

850

Approximate temperature

55°C

Reverse primer (5'-3')

ATCTTTCCAGCAGGAGATTTTGG

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

450

Solanum lycopersicum TA209

850

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 12097
Forward primer (5'-3') AGCAGGGGAGATTCCTATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATCTTTCCAGCAGGAGATTTTGG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 350	Approximate temperature 55°C

PCR data Exp. ID 12098
Forward primer (5'-3') AGCAGGGGAGATTCCTATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATCTTTCCAGCAGGAGATTTTGG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 350	Approximate temperature 55°C

PCR data Exp. ID 12099
Forward primer (5'-3') AGCAGGGGAGATTCCTATGGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATCTTTCCAGCAGGAGATTTTGG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 350	Approximate temperature 55°C

PCR data Exp. ID 12100
Forward primer (5'-3') AGCAGGGGAGATTCCTATGGC	Digested band sizes (using DpnII) Solanum lycopersicum LA925: 200 Solanum pennellii LA716: 180	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATCTTTCCAGCAGGAGATTTTGG	Enzymes All possible enzymes for pennellii and and lycopersicum: DpnII
Accessions and product sizes Solanum pennellii LA716: 350 Solanum lycopersicum LA925: 350	Approximate temperature 55°C