Sol Genomics Network

SGN-M9178
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	309353 (SGN) 128297 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U201136	-	-	-
Lycopersicon Combined	Single	SGN-U230532	-	-	-
Solanum tuberosum	Single	SGN-U247572	-	-	-
Arabidopsis	Single	At5g63460 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 40017

Forward primer (5'-3')

TTCTCGCGGCCTTTTCTCCTC

Accessions and product sizes

Solanum lycopersicum TA209

250

Solanum pennellii TA56

250

Approximate temperature

55°C

Reverse primer (5'-3')

TCGTGATCGCAAACATATACTCGC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

250

Solanum pennellii TA56

80+120

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 12101
Forward primer (5'-3') TTCTCGCGGCCTTTTCTCCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGTGATCGCAAACATATACTCGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1100	Approximate temperature 55°C

PCR data Exp. ID 12102
Forward primer (5'-3') TTCTCGCGGCCTTTTCTCCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGTGATCGCAAACATATACTCGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1100	Approximate temperature 55°C

PCR data Exp. ID 12103
Forward primer (5'-3') TTCTCGCGGCCTTTTCTCCTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGTGATCGCAAACATATACTCGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1100	Approximate temperature 55°C

PCR data Exp. ID 12104
Forward primer (5'-3') TTCTCGCGGCCTTTTCTCCTC	Digested band sizes (using HinfI) Solanum pennellii LA716: 850 Solanum lycopersicum LA925: 650	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGTGATCGCAAACATATACTCGC	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI, RsaI, HinfI, DpnII
Accessions and product sizes Solanum lycopersicum LA925: 1100 Solanum pennellii LA716: 1100	Approximate temperature 55°C