Publication: Naturally occurring broad-spectrum powdery mildew resistance in a central american tomato accession is caused by loss of mlo function.
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| Title | Naturally occurring broad-spectrum powdery mildew resistance in a central american tomato accession is caused by loss of mlo function. |
| Series name | Molecular plant-microbe interactions : MPMI |
| Volume | 21 |
| Issue | 1 |
| Year | 2008 |
| Pages | 30-9 |
| Authors | Bai, Yuling. Pavan, Stefano. Zheng, Zheng. Zappel, Nana. Reinstädler, Anja. Lotti, Concetta. De, Giovanni. Ricciardi, Luigi. Lindhout, Pim. Visser, Richard. Theres, Klaus. Panstruga, Ralph |
| Abstract | The resistant cherry tomato (Solanum lycopersicum var. cerasiforme) line LC-95, derived from an accession collected in Ecuador, harbors a natural allele (ol-2) that confers broad-spectrum and recessively inherited resistance to powdery mildew (Oidium neolycopersici). As both the genetic and phytopathological characteristics of ol-2-mediated resistance are reminiscent of powdery mildew immunity conferred by loss-of-function mlo alleles in barley and Arabidopsis, we initiated a candidate-gene approach to clone Ol-2. A tomato Mlo gene (SlMlo1) with high sequence-relatedness to barley Mlo and Arabidopsis AtMLO2 mapped to the chromosomal region harboring the Ol-2 locus. Complementation experiments using transgenic tomato lines as well as virus-induced gene silencing assays suggested that loss of SlMlo1 function is responsible for powdery mildew resistance conferred by ol-2. In progeny of a cross between a resistant line bearing ol-2 and the susceptible tomato cultivar Moneymaker, a 19-bp deletion disrupting the SlMlo1 coding region cosegregated with resistance. This polymorphism results in a frameshift and, thus, a truncated nonfunctional SlMlo1 protein. Our findings reveal the second example of a natural mlo mutant that possibly arose post-domestication, suggesting that natural mlo alleles might be evolutionarily short-lived due to fitness costs related to loss of mlo function. |
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