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Library Detail Page for FLMT

Short Name:FLMT
Organism:Solanum tuberosum
Library Name: Full Length Mixed Tuber
Total Sequences: 0 sequences from 0 clones
Average Sequence Length: 0.0 (Standard deviation 0.0)
Type:cDNA Library
Tissue:Mixed Tuber
Development Stage:
Treatment Conditions:supplier: Plants from pathogen-free Solanum tuberosum var. Shepody, clone 1756, nuclear stock were grown in a screenhouse under natural conditions. Stolons and mature tubers were used for total RNA isolations. Two hundred and fifty nanograms of each sample were pooled and used for full length 1st strand cDNA synthesis with a modified SMART cDNA kit (BD Biosciences), in which the kit CDS III primer was redesigned to contain an XhoI site. This was followed by PCR using the redesigned CDS III primer and the kit 5’ PCR primer. PCR products were blunted, and ligated to EcoRI adapters (Invitrogen), followed by XhoI digestion. The double stranded, digested cDNA was gel-purified, and the fraction above 650 bp was collected and ligated with the vector.
Cloning Host:XL10-Gold
Cloning Kit: Stratagene pBluescript II XR cDNA Library Construction Kit
Comments
AuthorsFlinn, B., Rothwell, C., Sardana, R., Griffiths, R.,\ Lague, M., De Koeyer, D., Audy, P., Goyer, C., Li, X-Q., Wang-Pruski, G., Regan, S.\
Contact InformationBarry Flinn\ bflinn@bioatlantech.nb.ca\ The Canadian Potato Genome Project - BioAtlantech Unit\ 921 College Hill Rd, Fredericton, ON, E3B 6Z9, CANADA\