Library Detail Page for PPI
| Short Name: | PPI |
| Organism: | Solanum tuberosum |
| Library Name: | P. infestans challenged Leaf (Incompatible) |
| Total Sequences: | 4918 sequences from 5434 clones |
| Average Sequence Length: | 612.0 (Standard deviation 120.0) |
| Type: | cDNA Library |
| Tissue: | leaf |
| Development Stage: | 6-week old leaf |
| Treatment Conditions: | Whole plants were challenged with 450,000 sporangia/ml P.infestans US-1 (US 940501) in Biotron (Madison, Wisconsin). Incompatible pathogen interaction, plants did not show HR. |
| Cloning Host: | E.coli SOLR |
| Cloning Kit: | |
| Comments | Leaf tissue was collected at 1, 2, 5, 12, and 24 hours post challenge and frozen in liquid nitrogen immediately upon removal. Kennebec plants showed no signs of HR. Katahdin plants (susceptible to P.infestans US-1) were used as controls and showed infection. See PPC library for compatible pathogen interaction. |
| Authors | Zhang, P., Hernandez, M., Tornqvist, C-E., Wirtz, U., Loukoianov, A., Rangel, P., Cho, J., Chiemingo, A., Bougri, O., Buell, C.R., Ronning, C.M., Baker, B. |
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